Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RA177_RS01900 Genome accession   NZ_CP132599
Coordinates   359414..359842 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus safensis strain PL23A     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 354414..364842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA177_RS01870 (RA177_01875) - 354577..354906 (+) 330 WP_185854516.1 YckD family protein -
  RA177_RS01875 (RA177_01880) - 354950..355714 (-) 765 WP_034624002.1 MBL fold metallo-hydrolase -
  RA177_RS01880 (RA177_01885) - 355830..356246 (+) 417 WP_025092811.1 Lrp/AsnC family transcriptional regulator -
  RA177_RS01885 (RA177_01890) - 356275..357081 (+) 807 WP_185854078.1 aminoglycoside N(3)-acetyltransferase -
  RA177_RS01890 (RA177_01895) - 357083..357469 (-) 387 WP_041106618.1 competence protein ComJ -
  RA177_RS18955 - 357589..358536 (-) 948 WP_370880873.1 methyl-accepting chemotaxis protein -
  RA177_RS18960 - 358528..359343 (-) 816 Protein_338 cache domain-containing protein -
  RA177_RS01900 (RA177_01905) nucA/comI 359414..359842 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RA177_RS01905 (RA177_01910) - 360210..362279 (+) 2070 WP_185854080.1 hydantoinase/oxoprolinase family protein -
  RA177_RS01910 (RA177_01915) - 362269..364257 (+) 1989 WP_185854081.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=869564 RA177_RS01900 WP_024424305.1 359414..359842(-) (nucA/comI) [Bacillus safensis strain PL23A]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=869564 RA177_RS01900 WP_024424305.1 359414..359842(-) (nucA/comI) [Bacillus safensis strain PL23A]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCATCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCAATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTGAGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATTCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535