Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RA224_RS00595 Genome accession   NZ_CP132598
Coordinates   124915..126360 (+) Length   481 a.a.
NCBI ID   WP_118934761.1    Uniprot ID   -
Organism   Achromobacter aegrifaciens strain L3024     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 119915..131360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA224_RS00580 (RA224_00580) - 120672..122000 (+) 1329 WP_118934766.1 amidase -
  RA224_RS00585 (RA224_00585) leuA 122067..123779 (-) 1713 WP_054455581.1 2-isopropylmalate synthase -
  RA224_RS00590 (RA224_00590) - 124299..124916 (-) 618 WP_118934763.1 hypothetical protein -
  RA224_RS00595 (RA224_00595) rpoN/rpoN1 124915..126360 (+) 1446 WP_118934761.1 RNA polymerase factor sigma-54 Machinery gene
  RA224_RS00600 (RA224_00600) - 126494..126775 (+) 282 WP_054455575.1 bacterioferritin-associated ferredoxin -
  RA224_RS00605 (RA224_00605) - 126806..127663 (-) 858 WP_306634844.1 LysR substrate-binding domain-containing protein -
  RA224_RS00610 (RA224_00610) - 127792..128706 (+) 915 WP_306634845.1 DMT family transporter -
  RA224_RS00615 (RA224_00615) bfr 129073..129549 (+) 477 WP_013390908.1 bacterioferritin -
  RA224_RS00620 (RA224_00620) - 129632..130318 (-) 687 WP_187524009.1 hypothetical protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52440.75 Da        Isoelectric Point: 4.7991

>NTDB_id=869546 RA224_RS00595 WP_118934761.1 124915..126360(+) (rpoN/rpoN1) [Achromobacter aegrifaciens strain L3024]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLAENPLLERQDEAPSAEAPAESDRESSVQDDEP
AAERETPVDEMPGSGGVYPDEDSSRPEAARPDTLREHLLGQLMLTRAAPRDAALAGLLIDELDENGYLGSPLEEILDWLP
PELEPDIDELKAALSLLQSFDPPGIGARDMAECLLLQLRNPDLDRLPEAADAAVLACARQICAQHLALLATGNAARLCAA
VGCDEATFRSAHALILRLEPRPGRAWTVPAADYAVPDVIVRKTRGGWQVTLNSAAVPKLQINGLYAQMLGNQKESAHAGL
QSQLQQARWMIRNVEQRFDTILRVAQAIVQHQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTDGGDATSATAVQTFIRQMVAEENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQAAA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=869546 RA224_RS00595 WP_118934761.1 124915..126360(+) (rpoN/rpoN1) [Achromobacter aegrifaciens strain L3024]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGACCAGGTCAGCATTTGACGCTGACGCCACAACTACA
GCAATCGATCCGATTGTTGCAGCTGTCCACGCTCGACCTCGAAGCCGAGATTTCGCAGGTGCTGGCGGAGAATCCGCTGC
TGGAGCGGCAGGACGAAGCGCCGTCGGCCGAGGCGCCGGCCGAGTCCGACCGCGAATCCTCGGTGCAGGATGACGAGCCC
GCCGCCGAACGCGAGACGCCGGTGGACGAAATGCCGGGCTCGGGCGGGGTCTACCCCGACGAAGATTCCAGCCGCCCCGA
GGCCGCCCGGCCGGATACGCTGCGCGAACATCTGCTGGGCCAGCTGATGCTGACGCGCGCCGCGCCGCGCGACGCTGCGC
TGGCCGGCCTCCTGATCGACGAGCTGGACGAAAACGGCTACCTGGGATCGCCGCTGGAGGAAATCCTGGACTGGTTGCCG
CCCGAACTGGAACCGGACATCGACGAGCTGAAGGCGGCCCTGTCGCTGTTGCAGTCGTTCGATCCGCCCGGCATAGGCGC
GCGCGACATGGCGGAATGCCTGTTGTTGCAGCTACGCAACCCGGATCTGGATCGGCTGCCCGAAGCGGCGGACGCCGCGG
TCCTGGCTTGTGCCCGCCAGATCTGCGCGCAGCACCTCGCGCTGCTGGCGACGGGCAACGCCGCGCGCCTGTGCGCGGCC
GTGGGCTGCGATGAGGCCACGTTCCGCTCGGCCCATGCCCTCATCCTGCGCCTGGAGCCCCGCCCCGGGCGTGCCTGGAC
CGTGCCCGCGGCCGACTACGCGGTGCCGGACGTCATCGTGCGCAAGACGCGCGGCGGCTGGCAGGTCACCCTCAACAGCG
CCGCCGTGCCCAAGCTGCAGATCAACGGGCTGTACGCCCAGATGCTGGGCAACCAGAAGGAATCCGCGCATGCGGGGCTG
CAATCGCAGCTGCAGCAGGCCCGTTGGATGATCCGCAACGTGGAGCAGCGCTTCGACACCATCCTGCGCGTGGCCCAAGC
CATCGTGCAGCACCAGACGGCCTTCTTCAGCCAGGGGCCGGCCGCCATGCGGCCTCTCATCCTGAAGGACATCGCCGGTG
AGCTCGGTTTGCACGAATCCACCATTTCACGGGCCACAACACAGAAGTACATGCTCACTCCGTTTGGCACGCTGGAGCTC
AAGCGCTTCTTTGGTACGGGGGTTTCGACGGATGGCGGGGATGCCACCTCGGCCACGGCGGTGCAGACCTTCATCCGCCA
GATGGTGGCCGAAGAGAACCGGGCCAAGCCTTTGTCCGACAGCCAGATCATGCAAAAGCTGGCTGATCAGGGGATAGTCA
TCGCCCGAAGGACGGTGGCAAAGTACCGGGAAGCGCTGCGGATTGCCCCGGCGGCGTTGCGCAAGGCGCAGGCGGCAGCC
CGCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

47.316

100

0.495