Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LACZS1_RS10995 Genome accession   NZ_CP132484
Coordinates   2361367..2362731 (-) Length   454 a.a.
NCBI ID   WP_010492253.1    Uniprot ID   A0AAD1ETX9
Organism   Lacticaseibacillus zeae subsp. silagei strain NCIMB 15474     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2356367..2367731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LACZS1_RS10975 (LACZS1_002191) - 2357269..2358045 (-) 777 WP_070650332.1 ABC transporter permease -
  LACZS1_RS10980 (LACZS1_002192) - 2358071..2358949 (-) 879 WP_070650333.1 ATP-binding cassette domain-containing protein -
  LACZS1_RS10985 (LACZS1_002193) - 2359230..2360126 (+) 897 WP_070650334.1 Rgg/GadR/MutR family transcriptional regulator -
  LACZS1_RS10990 (LACZS1_002194) - 2360231..2361346 (-) 1116 WP_010492251.1 PIN/TRAM domain-containing protein -
  LACZS1_RS10995 (LACZS1_002195) radA 2361367..2362731 (-) 1365 WP_010492253.1 DNA repair protein RadA Machinery gene
  LACZS1_RS11000 (LACZS1_002196) - 2362751..2363290 (-) 540 WP_070650335.1 hypothetical protein -
  LACZS1_RS11005 (LACZS1_002197) - 2363629..2363919 (+) 291 WP_070650336.1 GNAT family N-acetyltransferase -
  LACZS1_RS11010 (LACZS1_002198) - 2364378..2365697 (+) 1320 WP_070650337.1 aminopeptidase C -
  LACZS1_RS11015 (LACZS1_002199) - 2365828..2367174 (+) 1347 WP_070650338.1 C1 family peptidase -
  LACZS1_RS11020 (LACZS1_002200) - 2367306..2367671 (-) 366 WP_070650339.1 type II toxin-antitoxin system PemK/MazF family toxin -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49051.51 Da        Isoelectric Point: 8.0456

>NTDB_id=869262 LACZS1_RS10995 WP_010492253.1 2361367..2362731(-) (radA) [Lacticaseibacillus zeae subsp. silagei strain NCIMB 15474]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSTKSAPRQTRAGSKVKPMRMDEVTVAKETRVKTGLAELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLASTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIEGVI
DQMQPDYVVIDSVQTMNVPDMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQNGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEIPPTDCFVGEIGLTGEV
RRVNRIEDRVKEAAKLGFKRIFVPRNNLQGWHAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=869262 LACZS1_RS10995 WP_010492253.1 2361367..2362731(-) (radA) [Lacticaseibacillus zeae subsp. silagei strain NCIMB 15474]
ATGGCTAAAGCAAAAACACAGTACGTATGTCAAAATTGCGGTTATATTTCGGCCACTTATTTAGGGCGTTGTCCGAATTG
TGGCGGCTGGAATACCTTAGTTGAAGAAACCGTCAGTTCAACCAAGTCGGCACCGCGGCAAACGCGGGCAGGCAGTAAAG
TCAAGCCGATGCGGATGGATGAAGTGACGGTTGCCAAGGAAACGCGGGTTAAGACCGGTTTGGCGGAATTAAACCGTGTG
CTTGGTGGCGGCGTGGTGCCGGGATCGCTGGTGTTGATTGGCGGGGATCCTGGCATCGGTAAATCGACCCTGTTGTTGCA
GGTGTCCGGGCAGTTGGCAAGCACTGGCGGCACCGTTTTATACGTGTCCGGCGAAGAAAGTGCCAGTCAGATCAAAATGC
GTGCCGGCCGCTTAGGGGTTGCCAATTCGGGGATGTACCTGTATCCGGAAACCGACATGCCCAGCATTGAAGGCGTTATT
GACCAGATGCAGCCGGATTATGTCGTGATTGATTCGGTGCAAACCATGAATGTACCGGATATGAACTCGGCAGTCGGTTC
GGTTGCCCAGATTCGCGAAGTTACAGCCGAACTGATGCGCATTGCCAAATCTAAAGGCGTGACGATCTTCATCGTTGGTC
ACGTGACTAAGGAAGGTGCCATTGCCGGCCCTAAGATTCTTGAGCATATGGTCGACACTGTGCTTTATTTTGAAGGCGAT
ATGCACCACACTTACCGGATTTTACGGTCGGTTAAGAATCGGTTCGGCTCTACCAATGAAATCGGCATTTTTGAGATGCA
CCAAAATGGCCTGCAGGAAGTTGCCAATCCCTCGGAAATCTTCTTAGAAGAACGGCTGGCTGGGGCAACCGGTTCGGCGG
TGGTGGTGTCGATGGAAGGTACGCGGCCGATTTTGGTGGAAATTCAGGCATTGATCAGTCCCACGATGTACGGCAATGCC
AAACGCACCAGCAGCGGATTGGATCACAATCGCGTCAGCTTGATCATGGCAGTGCTGGAAAAACGGGCCAATCTCATGCT
GCAGAATCAGGACGCTTACCTGAAAGCCACTGGCGGCGTTAAGTTGGATGAACCGGCGATTGACTTAGCAATGGCCGTTG
CGATTGCCTCGTCATATCGCGACAAAGAGATTCCGCCGACGGATTGTTTTGTCGGTGAAATCGGCCTGACCGGTGAAGTG
CGGCGGGTGAACCGCATTGAAGATCGGGTCAAAGAGGCGGCCAAGCTGGGTTTTAAGCGGATTTTCGTACCGCGCAACAA
CCTGCAAGGCTGGCACGCACCCAAAGATATTCAAGTCATTGGGGTTACAAGCATTGCCGAAGCGTTGCATAAGGTTTTTA
ATTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(69-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.433

99.78

0.683
  radA Streptococcus pneumoniae Rx1

68.433

99.78

0.683
  radA Streptococcus pneumoniae D39

68.433

99.78

0.683
  radA Streptococcus pneumoniae R6

68.433

99.78

0.683
  radA Streptococcus pneumoniae TIGR4

68.433

99.78

0.683
  radA Streptococcus mitis SK321

68.212

99.78

0.681
  radA Bacillus subtilis subsp. subtilis str. 168

65.132

100

0.654