Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RAM40_RS13035 Genome accession   NZ_CP132400
Coordinates   2678658..2680028 (-) Length   456 a.a.
NCBI ID   WP_014614769.1    Uniprot ID   A0A166QVY3
Organism   Staphylococcus pseudintermedius strain CUVET16-496     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2673658..2685028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM40_RS13010 (RAM40_12905) cysE 2673823..2674488 (-) 666 WP_172425792.1 serine O-acetyltransferase -
  RAM40_RS13015 (RAM40_12910) gltX 2674876..2676330 (-) 1455 WP_037543943.1 glutamate--tRNA ligase -
  RAM40_RS13020 (RAM40_12915) ispF 2676407..2676886 (-) 480 WP_014614766.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  RAM40_RS13025 (RAM40_12920) ispD 2676879..2677577 (-) 699 WP_014614767.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  RAM40_RS13030 (RAM40_12925) - 2677578..2678639 (-) 1062 WP_014614768.1 PIN/TRAM domain-containing protein -
  RAM40_RS13035 (RAM40_12930) radA 2678658..2680028 (-) 1371 WP_014614769.1 DNA repair protein RadA Machinery gene
  RAM40_RS13040 (RAM40_12935) - 2680415..2681161 (-) 747 WP_014614770.1 ABC transporter permease -
  RAM40_RS13045 (RAM40_12940) - 2681148..2681852 (-) 705 WP_014614771.1 ABC transporter ATP-binding protein -
  RAM40_RS13050 (RAM40_12945) - 2681867..2682826 (-) 960 WP_099986825.1 hypothetical protein -
  RAM40_RS13055 (RAM40_12950) - 2682826..2683638 (-) 813 WP_065520705.1 SagB family peptide dehydrogenase -
  RAM40_RS13060 (RAM40_12955) - 2683658..2684878 (-) 1221 WP_100002356.1 YcaO-like family protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49994.48 Da        Isoelectric Point: 7.0051

>NTDB_id=869141 RAM40_RS13035 WP_014614769.1 2678658..2680028(-) (radA) [Staphylococcus pseudintermedius strain CUVET16-496]
MAKTKTIFECTACGYQSPKWMGKCPNCGAWNSMEESFEQKTASPKHGVRAQKTSSAKIQKLDDIKQELAPRIQTNSGELN
RVLGGGIVEGSLVLIGGDPGIGKSTLLLQMCAALSKEKRVLYITGEESLNQTKIRADRLDEDASQLNVFAETDLMVIHEA
VKKVEPDLIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNVATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKSVLNPSEMFLEERTTNVAGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLGIVIATASSFKDQAVDGLDCYIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWHFPEGIEVIGVTSVNEALKYALKN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=869141 RAM40_RS13035 WP_014614769.1 2678658..2680028(-) (radA) [Staphylococcus pseudintermedius strain CUVET16-496]
GTGGCAAAAACAAAAACAATTTTTGAATGTACGGCCTGTGGTTATCAATCGCCTAAATGGATGGGGAAATGTCCAAATTG
TGGTGCTTGGAATTCAATGGAGGAATCTTTTGAACAAAAAACTGCGAGTCCTAAACACGGTGTACGGGCGCAAAAAACAA
GTTCAGCAAAAATACAAAAGCTCGATGACATTAAACAAGAGCTTGCGCCACGTATCCAAACGAATAGTGGTGAATTAAAC
CGTGTGCTCGGTGGCGGTATTGTAGAAGGGTCACTCGTGCTGATTGGTGGCGATCCAGGAATAGGGAAGTCCACATTGTT
ACTTCAAATGTGCGCGGCTTTATCTAAAGAGAAAAGGGTGCTCTATATTACTGGTGAGGAATCACTCAATCAAACTAAAA
TTAGAGCGGATCGTTTGGATGAAGATGCGAGTCAACTTAACGTGTTTGCGGAAACGGACTTAATGGTTATTCATGAAGCG
GTGAAAAAGGTCGAACCTGATTTAATTGTAGTGGACTCTATTCAGACGATCTACCATCCCGAAATCAGTTCGGCGCCTGG
CTCTGTATCTCAAGTTCGTGAAAGTACACAAAGCTTGATGCACATCGCAAAACAAATGAATGTTGCGACGTTTATTGTAG
GCCATGTGACTAAAGAAGGCCAAATCGCGGGGCCGCGTTTACTTGAACATATGGTGGATACGGTGCTTTATTTTGAAGGT
GATGAACATCATGCGTATCGTATTTTAAGAGCGGTGAAAAACCGTTTTGGCTCTACGAATGAAATGGGTATTTTTGAAAT
GAAACAGTCAGGTTTAAAAAGCGTGCTTAACCCGTCAGAAATGTTTTTAGAAGAACGGACGACCAATGTTGCAGGTTCGA
CAATTGTCGCAACGATGGAAGGGACACGACCATTGTTAATTGAAGTACAAGCGCTTGTCACACCGACGACGTTCAATAAT
CCGAGACGTATGGCGACAGGTATTGACCATAATCGCCTCAGTTTGTTAATGGCGGTGTTAGAGAAAAAAGAAGGTTATTT
ATTACAACAACAAGACGCTTACATTAAGGTGGCTGGGGGCGTCAAACTCTCTGAACCTGCTGTCGACTTAGGTATCGTCA
TTGCAACAGCTTCTAGTTTTAAAGACCAAGCAGTAGACGGCCTCGATTGTTATATTGGCGAGGTTGGATTGACTGGTGAG
GTCCGCCGTGTTTCGCGCATTGAGCAACGTGTGCAAGAAGCAGCAAAATTGGGCTTTAAACGTGTGATTATCCCTAAAAA
CAATATTGGCGGATGGCACTTCCCAGAAGGTATTGAAGTGATTGGCGTGACTTCTGTCAATGAAGCATTGAAATACGCAT
TAAAAAATTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-220)

Subunit ChlI of Mg-chelatase (ChlI)

(345-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A166QVY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.789

100

0.658
  radA Streptococcus pneumoniae Rx1

58.628

99.123

0.581
  radA Streptococcus pneumoniae D39

58.628

99.123

0.581
  radA Streptococcus pneumoniae R6

58.628

99.123

0.581
  radA Streptococcus pneumoniae TIGR4

58.628

99.123

0.581
  radA Streptococcus mitis NCTC 12261

58.628

99.123

0.581
  radA Streptococcus mitis SK321

58.628

99.123

0.581