Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABJ73_RS22300 Genome accession   NZ_AP027520
Coordinates   4541796..4542473 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain LR-31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4536796..4547473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ73_RS22265 (VEE27_42920) gspG 4537276..4537713 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  AABJ73_RS22270 (VEE27_42930) gspH 4537721..4538230 (+) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  AABJ73_RS22275 (VEE27_42940) gspI 4538227..4538604 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AABJ73_RS22280 (VEE27_42950) gspJ 4538597..4539184 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  AABJ73_RS22285 (VEE27_42960) gspK 4539177..4540160 (+) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  AABJ73_RS22290 (VEE27_42970) gspL 4540175..4541338 (+) 1164 WP_001115228.1 type II secretion system protein GspL -
  AABJ73_RS22295 (VEE27_42980) gspM 4541335..4541796 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  AABJ73_RS22300 (VEE27_42990) pilD 4541796..4542473 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  AABJ73_RS22305 (VEE27_43000) bfr 4542502..4542978 (-) 477 WP_000675504.1 bacterioferritin -
  AABJ73_RS22310 (VEE27_43010) bfd 4543050..4543244 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABJ73_RS22315 (VEE27_43020) chiA 4543413..4546106 (-) 2694 WP_000773143.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=86893 AABJ73_RS22300 WP_000178161.1 4541796..4542473(+) (pilD) [Escherichia coli strain LR-31]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=86893 AABJ73_RS22300 WP_000178161.1 4541796..4542473(+) (pilD) [Escherichia coli strain LR-31]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment