Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RAM07_RS06745 Genome accession   NZ_CP132379
Coordinates   1406904..1408280 (-) Length   458 a.a.
NCBI ID   WP_306268201.1    Uniprot ID   -
Organism   Lactobacillus helsingborgensis strain K-FP18     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1401904..1413280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM07_RS06720 (RAM07_06725) - 1402485..1402928 (-) 444 WP_306268191.1 Mini-ribonuclease 3 -
  RAM07_RS06725 - 1403152..1403346 (-) 195 WP_306268193.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
  RAM07_RS06730 (RAM07_06735) - 1403343..1403639 (-) 297 WP_306268195.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  RAM07_RS06735 (RAM07_06740) cysS 1403744..1405168 (-) 1425 WP_306268197.1 cysteine--tRNA ligase -
  RAM07_RS06740 (RAM07_06745) gltX 1405330..1406829 (-) 1500 WP_306268199.1 glutamate--tRNA ligase -
  RAM07_RS06745 (RAM07_06750) radA 1406904..1408280 (-) 1377 WP_306268201.1 DNA repair protein RadA Machinery gene
  RAM07_RS06750 (RAM07_06755) - 1408281..1408832 (-) 552 WP_306268203.1 dUTP diphosphatase -
  RAM07_RS06755 (RAM07_06760) - 1408946..1409260 (+) 315 WP_306268205.1 hypothetical protein -
  RAM07_RS06760 (RAM07_06765) pepC 1409390..1410736 (+) 1347 WP_306268207.1 aminopeptidase C -
  RAM07_RS06765 (RAM07_06770) galT 1410857..1412335 (-) 1479 WP_306268210.1 UDP-glucose--hexose-1-phosphate uridylyltransferase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49962.76 Da        Isoelectric Point: 7.6993

>NTDB_id=868854 RAM07_RS06745 WP_306268201.1 1406904..1408280(-) (radA) [Lactobacillus helsingborgensis strain K-FP18]
MAKLKTKYKCRSCGYISASYLGRCPNCGAWNQFEKETEAVQNRSTKGSPSRLIKKTGISEPVKLGNVKAEKEERIKTSMA
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSELADQYKVLYVSGEESANQIKLRADRLGLPANDMMLYPETDMEDI
RQQIADLEPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHAYRILHSVKNRFGAANEIGMFEMVNKGLQEVTNPSAVFLDERLPKSTGSAIVVSLEGTRPILAEIQALVTPTA
FGYAKRTTSGIDYNRAGLLLAVLEKRGNLMLQNQDVYLTATGGIRLNEPAVDLAVVMAIASSYKNQEILPTDCFVGEVGL
TGEVRRVNQIDARIKEASKTGFKRIFIPKHNMRASLKNSGIEVIPVASIPQALKVVLG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=868854 RAM07_RS06745 WP_306268201.1 1406904..1408280(-) (radA) [Lactobacillus helsingborgensis strain K-FP18]
ATGGCAAAACTAAAAACAAAATATAAATGCCGTTCTTGTGGTTATATTTCTGCCAGCTATTTAGGGCGCTGTCCTAATTG
CGGTGCTTGGAATCAGTTTGAAAAAGAAACTGAAGCAGTGCAAAACCGTTCAACTAAAGGCAGTCCCAGCAGGCTCATCA
AGAAAACCGGAATTAGCGAGCCGGTTAAATTGGGGAATGTGAAGGCTGAAAAAGAAGAGCGCATCAAAACAAGTATGGCG
GAACTGAACAGGGTTTTAGGCGGCGGTATTGTTCCCGGTTCACTGGTTTTAATCGGTGGTGATCCTGGAATCGGTAAGTC
AACTTTAATGTTACAAATCATGAGCGAATTGGCTGATCAATATAAAGTGCTGTATGTTTCAGGGGAAGAATCTGCTAATC
AAATCAAGCTCCGTGCAGATCGCTTAGGTCTTCCTGCTAATGACATGATGCTTTATCCAGAAACAGATATGGAAGATATT
CGTCAGCAAATTGCTGATTTAGAACCCGATTTTGTCGTGATTGACTCCATTCAGACCATGAATGAACCAAGTCTTGACTC
GATGACCGGTTCGGCGTCACAAGTGAGGGAAGTCACAAGCGAATTGATGAAAATCGCTAAAATGGATGCGATTACCGTTT
TTGTTATTGGGCACGTGACAAAAGAAGGTGCAATTGCAGGACCTAAAATTCTGGAGCACATGGTAGATACAGTCCTTTAT
TTTGAAGGTGATGAACATCATGCCTACCGTATTTTACATTCTGTTAAAAATCGGTTTGGTGCTGCAAATGAAATCGGCAT
GTTTGAAATGGTTAACAAAGGTTTGCAGGAAGTAACTAACCCTTCAGCCGTATTCTTAGATGAACGGTTGCCCAAATCTA
CCGGTTCGGCGATAGTAGTATCGCTTGAAGGAACAAGGCCAATTTTGGCGGAAATTCAGGCATTAGTGACACCGACTGCT
TTTGGTTATGCAAAAAGAACCACTTCGGGTATTGACTACAATAGAGCTGGATTATTGCTAGCTGTCTTGGAAAAAAGAGG
CAACTTGATGCTGCAAAATCAAGACGTTTATCTAACTGCCACTGGGGGAATTCGTTTGAACGAACCGGCAGTTGATTTGG
CAGTAGTCATGGCAATTGCCTCAAGTTATAAAAATCAGGAAATTTTGCCTACGGACTGTTTTGTGGGCGAAGTCGGCTTA
ACTGGTGAGGTACGCAGAGTCAATCAAATAGATGCGCGTATTAAAGAAGCTTCTAAGACTGGTTTCAAACGTATTTTTAT
TCCGAAACACAATATGCGTGCTAGTCTTAAAAACTCGGGAATTGAAGTGATTCCAGTTGCAAGTATTCCGCAAGCCTTAA
AAGTGGTTTTAGGGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-223)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

58.297

100

0.583
  radA Streptococcus pneumoniae Rx1

58.297

100

0.583
  radA Streptococcus pneumoniae D39

58.297

100

0.583
  radA Streptococcus pneumoniae R6

58.297

100

0.583
  radA Streptococcus pneumoniae TIGR4

58.297

100

0.583
  radA Streptococcus mitis SK321

58.297

100

0.583
  radA Bacillus subtilis subsp. subtilis str. 168

56.399

100

0.568