Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RAM13_RS05605 Genome accession   NZ_CP132378
Coordinates   1135699..1137075 (-) Length   458 a.a.
NCBI ID   WP_046307452.1    Uniprot ID   A0A3M0NHC6
Organism   Lactobacillus apis strain K-MP7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1130699..1142075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM13_RS05585 (RAM13_05585) rlmB 1131368..1132123 (-) 756 WP_306267323.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  RAM13_RS05590 (RAM13_05590) - 1132110..1132553 (-) 444 WP_122035694.1 Mini-ribonuclease 3 -
  RAM13_RS05595 (RAM13_05595) cysS 1132546..1133970 (-) 1425 WP_306265501.1 cysteine--tRNA ligase -
  RAM13_RS05600 (RAM13_05600) gltX 1134118..1135623 (-) 1506 WP_074426298.1 glutamate--tRNA ligase -
  RAM13_RS05605 (RAM13_05605) radA 1135699..1137075 (-) 1377 WP_046307452.1 DNA repair protein RadA Machinery gene
  RAM13_RS05610 (RAM13_05610) - 1137076..1137624 (-) 549 WP_046307454.1 dUTP diphosphatase -
  RAM13_RS05615 (RAM13_05615) - 1137824..1138132 (+) 309 WP_306265506.1 hypothetical protein -
  RAM13_RS05620 (RAM13_05620) pepC 1138278..1139624 (+) 1347 WP_046307458.1 aminopeptidase C -
  RAM13_RS05625 (RAM13_05625) - 1139684..1140619 (-) 936 WP_306265509.1 AAA family ATPase -
  RAM13_RS05630 (RAM13_05630) - 1140725..1141399 (-) 675 WP_306265511.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
  RAM13_RS05635 (RAM13_05635) - 1141402..1141584 (-) 183 WP_306265514.1 hypothetical protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49977.82 Da        Isoelectric Point: 7.1405

>NTDB_id=868838 RAM13_RS05605 WP_046307452.1 1135699..1137075(-) (radA) [Lactobacillus apis strain K-MP7]
MAKIKTKYKCHSCGYISASYLGRCPNCGAWNQFEKETETVQAHSAKGSPSRLIKKTGINEPVKIEAVKAEKEERIQTSMG
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSELSQKFKVLYVSGEESANQIKLRADRLGLAANDMLLYPETDMEDI
RQQIDTIKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHAYRILHSVKNRFGAANEIGMFEMVNKGLQEVTNPSAIFLDERLPNSTGSAIVVSLEGTRPILAEIQALVTPTA
FGYAKRTTAGIDFNRAGLLLAVLEKRGNLMLQNQDVYLTATGGIRLNEPAVDLAVVMAIASSYKNQEILPTDCFVGEVGL
TGEVRRVNQIDARIKEAAKTGFKRIFIPKHNMRSSLQNMGIEVIPVSSIPQALKLVLA

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=868838 RAM13_RS05605 WP_046307452.1 1135699..1137075(-) (radA) [Lactobacillus apis strain K-MP7]
ATGGCCAAAATTAAAACGAAATATAAGTGCCACTCCTGTGGCTATATTTCGGCTAGCTACTTAGGAAGGTGTCCTAATTG
CGGTGCCTGGAATCAGTTTGAAAAAGAAACCGAAACTGTCCAAGCACATTCTGCAAAAGGTAGTCCAAGTAGACTAATTA
AGAAAACTGGAATTAATGAACCAGTTAAAATTGAAGCCGTAAAGGCTGAAAAAGAGGAACGAATCCAAACAAGTATGGGT
GAACTTAACCGTGTTCTTGGTGGCGGAATAGTTCCTGGCTCGCTTGTGTTAATCGGTGGAGACCCTGGAATTGGTAAGTC
TACTTTAATGTTACAAATCATGAGTGAGCTATCCCAAAAGTTCAAAGTCCTATACGTTTCGGGAGAAGAATCGGCTAATC
AAATCAAGTTGCGCGCAGATCGTCTAGGACTTGCTGCGAATGATATGTTGCTTTATCCAGAAACAGACATGGAAGACATT
CGCCAGCAGATTGACACAATCAAGCCTGATTTTGTCGTAATTGACTCAATTCAAACAATGAATGAACCTAGTCTTGATTC
AATGACGGGTTCCGCATCACAGGTTCGTGAAGTAACCAGCGAGTTAATGAAAATTGCTAAGATGGATGCAATTACGGTTT
TCGTGATCGGTCATGTTACCAAGGAAGGTGCAATTGCTGGGCCTAAGATTTTGGAACACATGGTGGATACAGTTCTTTAT
TTTGAAGGTGATGAACACCATGCGTACCGAATCTTGCATTCCGTAAAAAATCGGTTCGGTGCTGCTAACGAAATCGGTAT
GTTTGAAATGGTCAACAAAGGGTTGCAGGAAGTCACCAATCCATCTGCAATTTTTCTTGATGAGCGATTACCTAACTCAA
CGGGATCTGCGATTGTCGTCTCGCTTGAGGGAACAAGGCCGATTCTAGCGGAAATCCAAGCATTAGTTACACCGACCGCA
TTTGGCTACGCCAAAAGGACTACTGCCGGTATTGATTTCAACCGAGCAGGATTATTGTTAGCAGTTTTAGAAAAGCGTGG
CAACTTGATGTTACAAAATCAAGATGTTTATCTAACTGCTACCGGTGGTATTCGCTTGAATGAACCTGCAGTTGATTTGG
CGGTAGTGATGGCGATTGCTTCAAGCTATAAAAACCAAGAAATTTTACCAACTGATTGCTTCGTGGGTGAGGTTGGTTTA
ACTGGTGAAGTTAGACGAGTTAACCAAATTGATGCGCGGATTAAAGAAGCAGCTAAAACAGGTTTTAAGCGAATTTTTAT
TCCTAAGCATAATATGCGCTCAAGCTTGCAAAATATGGGAATAGAAGTAATTCCTGTTTCAAGCATCCCCCAAGCGTTAA
AATTGGTTTTGGCTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-223)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M0NHC6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

59.607

100

0.596
  radA Streptococcus mitis NCTC 12261

59.389

100

0.594
  radA Streptococcus pneumoniae Rx1

59.389

100

0.594
  radA Streptococcus pneumoniae D39

59.389

100

0.594
  radA Streptococcus pneumoniae R6

59.389

100

0.594
  radA Streptococcus pneumoniae TIGR4

59.389

100

0.594
  radA Bacillus subtilis subsp. subtilis str. 168

55.991

100

0.561