Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RAM23_RS01310 Genome accession   NZ_CP132374
Coordinates   293342..294637 (-) Length   431 a.a.
NCBI ID   WP_100126046.1    Uniprot ID   -
Organism   Snodgrassella alvi strain wkB339     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 288342..299637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM23_RS01295 (RAM23_01295) - 289714..291414 (+) 1701 WP_180296361.1 McrB family protein -
  RAM23_RS01300 (RAM23_01300) - 291435..292553 (+) 1119 WP_100126047.1 5-methylcytosine-specific restriction endonuclease system specificity protein McrC -
  RAM23_RS01305 (RAM23_01305) - 292588..293121 (-) 534 WP_084565208.1 hypothetical protein -
  RAM23_RS01310 (RAM23_01310) pilA 293342..294637 (-) 1296 WP_100126046.1 signal recognition particle-docking protein FtsY Machinery gene
  RAM23_RS01315 (RAM23_01315) - 294853..295467 (-) 615 WP_100126045.1 Type 1 glutamine amidotransferase-like domain-containing protein -
  RAM23_RS01320 (RAM23_01320) - 295512..296063 (-) 552 WP_100126044.1 5'-3'-deoxyribonucleotidase -
  RAM23_RS01325 (RAM23_01325) ruvA 296107..296688 (-) 582 WP_025331789.1 Holliday junction branch migration protein RuvA -
  RAM23_RS01330 (RAM23_01330) - 296766..297515 (-) 750 WP_280522358.1 septal ring lytic transglycosylase RlpA family protein -
  RAM23_RS01335 (RAM23_01335) - 297842..298552 (-) 711 WP_238791959.1 ComF family protein -
  RAM23_RS01340 (RAM23_01340) bioH 298551..299303 (+) 753 WP_100126042.1 pimeloyl-ACP methyl ester esterase BioH -

Sequence

Protein


Download         Length: 431 a.a.        Molecular weight: 46274.60 Da        Isoelectric Point: 4.4640

>NTDB_id=868730 RAM23_RS01310 WP_100126046.1 293342..294637(-) (pilA) [Snodgrassella alvi strain wkB339]
MFGFFKRKKNTEPVAEQPVPAPHQQENSEQLQNIADDSVQTEVIPQPEPAISDPVAEKPEQADTAPVQPQAETEKDIIVP
TAVDEPAAIAVTDDTASPVQTPVANENTVVIAAEPDTESEQTEKPAKLSWTARLKQGLSKSRNQMAKSLASVFGGGKIDE
DLYEELETVLLTSDMGIEATEKLLAQVRQRVTLKGLKNGDELRSALKEALYELLLPLDQPLILPDDKKPFVIMMAGVNGA
GKTTSIGKLAKYFQSQGKSVLLAAGDTFRAAAREQLQQWGERNNVTVIAQASGDSAAVCYDALEAAKARGIDVVLADTAG
RLPTQLHLMEEIKKVKRVLQKSLPDAPHEVMLVLDANVGQNAINQVVAFDDALGLTGLIVSKLDGTAKGGILAALAAKRP
VPVRFIGVGEGIDDLRPFDARAFVDALIEDE

Nucleotide


Download         Length: 1296 bp        

>NTDB_id=868730 RAM23_RS01310 WP_100126046.1 293342..294637(-) (pilA) [Snodgrassella alvi strain wkB339]
ATGTTTGGATTTTTTAAGCGAAAAAAAAATACTGAGCCAGTTGCTGAGCAGCCCGTACCGGCCCCTCACCAGCAGGAAAA
CTCAGAACAATTACAGAATATAGCTGATGATTCTGTTCAGACTGAAGTAATACCGCAGCCTGAACCGGCTATCAGTGATC
CAGTTGCAGAAAAACCTGAGCAGGCCGACACCGCTCCCGTTCAACCACAGGCCGAAACGGAAAAAGATATTATTGTACCC
ACAGCAGTAGATGAACCGGCAGCCATTGCTGTAACAGACGATACTGCTTCACCAGTTCAGACACCTGTCGCTAATGAAAA
CACAGTTGTGATAGCTGCTGAGCCGGATACAGAGAGTGAGCAGACAGAAAAACCGGCTAAACTAAGCTGGACTGCACGGC
TAAAACAGGGGCTGAGTAAATCCCGCAATCAAATGGCCAAATCTCTGGCTTCTGTATTTGGTGGCGGTAAAATCGACGAA
GATTTGTATGAAGAACTGGAAACCGTATTGCTTACCAGTGATATGGGAATTGAAGCCACAGAAAAACTGCTGGCACAGGT
GCGTCAGCGGGTTACCCTGAAAGGGCTGAAAAACGGAGATGAGCTGCGCAGTGCTCTGAAAGAAGCCCTGTATGAATTAC
TGTTGCCACTGGATCAGCCACTGATTTTACCGGATGATAAAAAGCCCTTTGTGATTATGATGGCCGGTGTGAATGGTGCC
GGTAAAACTACCAGTATCGGTAAACTGGCGAAATATTTTCAAAGTCAGGGTAAAAGCGTGCTGCTGGCTGCCGGCGATAC
CTTTCGTGCAGCAGCCCGCGAACAATTGCAGCAATGGGGTGAGCGCAATAATGTCACCGTGATTGCTCAGGCTTCCGGCG
ACTCAGCAGCAGTCTGTTATGATGCTCTGGAGGCGGCCAAAGCACGCGGTATTGATGTGGTACTGGCAGACACTGCCGGT
CGCCTGCCTACTCAGCTGCATCTGATGGAAGAAATCAAAAAAGTAAAACGCGTTCTGCAAAAATCCTTGCCTGATGCTCC
GCATGAAGTCATGCTGGTACTGGATGCCAATGTGGGTCAGAATGCCATCAATCAGGTTGTAGCCTTTGATGATGCACTCG
GATTAACCGGTCTGATTGTTTCCAAACTGGATGGTACAGCCAAAGGCGGTATTCTCGCGGCACTGGCAGCCAAGCGACCG
GTTCCGGTACGCTTTATCGGCGTGGGTGAAGGTATTGATGATTTACGCCCGTTTGATGCCCGAGCATTTGTAGATGCATT
AATTGAGGATGAATAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(145-210)

SRP54-type protein, GTPase domain (SRP54)

(229-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

63.004

100

0.652