Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   RAM34_RS11425 Genome accession   NZ_CP132365
Coordinates   2282587..2284215 (-) Length   542 a.a.
NCBI ID   WP_001186608.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UNC_SA54     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 2275822..2290757 2282587..2284215 within 0


Gene organization within MGE regions


Location: 2275822..2290757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM34_RS11390 (RAM34_11375) mecA 2275822..2277831 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  RAM34_RS11395 (RAM34_11380) - 2277928..2278914 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  RAM34_RS11400 (RAM34_11385) - 2278904..2279146 (+) 243 Protein_2206 type I restriction enzyme endonuclease domain-containing protein -
  RAM34_RS11405 (RAM34_11390) - 2279137..2280747 (-) 1611 Protein_2207 IS1182 family transposase -
  RAM34_RS11410 (RAM34_11395) - 2280796..2281302 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  RAM34_RS11415 (RAM34_11400) - 2281317..2281628 (-) 312 WP_000700851.1 DUF960 family protein -
  RAM34_RS11420 (RAM34_11405) - 2281715..2282065 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  RAM34_RS11425 (RAM34_11410) ccrB 2282587..2284215 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  RAM34_RS11430 (RAM34_11415) ccrA 2284237..2285586 (-) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  RAM34_RS11435 (RAM34_11420) cch1 2285820..2287613 (-) 1794 WP_024937223.1 cassette chromosome replicative helicase -
  RAM34_RS11440 (RAM34_11425) ssb 2287613..2287909 (-) 297 WP_000273823.1 cassette chromosome ssDNA-binding protein -
  RAM34_RS11445 (RAM34_11430) - 2288102..2289148 (-) 1047 WP_001042023.1 hypothetical protein -
  RAM34_RS11450 (RAM34_11435) - 2289603..2290757 (+) 1155 WP_000912116.1 ATP-binding protein -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62654.83 Da        Isoelectric Point: 9.7970

>NTDB_id=868558 RAM34_RS11425 WP_001186608.1 2282587..2284215(-) (ccrB) [Staphylococcus aureus strain UNC_SA54]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKTLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=868558 RAM34_RS11425 WP_001186608.1 2282587..2284215(-) (ccrB) [Staphylococcus aureus strain UNC_SA54]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAACAGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATCGACTGTGTTATG
GTATACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTGTCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAGATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAACGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTACCACTAGGTTATGACAAAATACCAGATAGTAAACACGAGCTAATGATTAACCAACATGAAGCTAATATTGTAAAATA
TATATTCGAGTGCTATGCCAAAGGACATGGCTATCGTAAAATTGCCAATGCATTGAATCACAAAGGATATGTCACTAAAA
AGGGGAAACCTTTCAGTATTAGTTCAATCACATACATCTTAGCTAACCCTTTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAGAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACTCTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAAATAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGACTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(180-280)

Resolvase, N terminal domain (Resolvase)

(11-156)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

98.893

100

0.989