Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   RAM32_RS11595 Genome accession   NZ_CP132361
Coordinates   2358457..2358954 (-) Length   165 a.a.
NCBI ID   WP_029694050.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UNC_SA56     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2353457..2363954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM32_RS11570 (RAM32_11560) gcvPB 2353619..2355091 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  RAM32_RS11575 (RAM32_11565) gcvPA 2355084..2356430 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  RAM32_RS11580 (RAM32_11570) gcvT 2356450..2357541 (-) 1092 WP_000093345.1 glycine cleavage system aminomethyltransferase GcvT -
  RAM32_RS11585 (RAM32_11575) - 2357700..2358224 (-) 525 WP_001015121.1 shikimate kinase -
  RAM32_RS11590 (RAM32_11580) - 2358214..2358360 (-) 147 WP_001789879.1 hypothetical protein -
  RAM32_RS11595 (RAM32_11585) comGF 2358457..2358954 (-) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  RAM32_RS11600 (RAM32_11590) comGE 2358872..2359171 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  RAM32_RS11605 (RAM32_11595) comGD 2359158..2359604 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  RAM32_RS11610 (RAM32_11600) comGC 2359582..2359893 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RAM32_RS11615 (RAM32_11605) comGB 2359907..2360977 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  RAM32_RS11620 (RAM32_11610) comGA 2360949..2361923 (-) 975 WP_000697219.1 competence type IV pilus ATPase ComGA Machinery gene
  RAM32_RS11625 (RAM32_11615) - 2361975..2362598 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RAM32_RS11630 (RAM32_11620) - 2362595..2362924 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RAM32_RS11635 (RAM32_11625) - 2362924..2363910 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19199.92 Da        Isoelectric Point: 10.2192

>NTDB_id=868453 RAM32_RS11595 WP_029694050.1 2358457..2358954(-) (comGF) [Staphylococcus aureus strain UNC_SA56]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=868453 RAM32_RS11595 WP_029694050.1 2358457..2358954(-) (comGF) [Staphylococcus aureus strain UNC_SA56]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97