Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RAK14_RS00330 Genome accession   NZ_CP132330
Coordinates   75202..76641 (+) Length   479 a.a.
NCBI ID   WP_010927240.1    Uniprot ID   A0A0H3LRZ4
Organism   Bordetella bronchiseptica strain XX35     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 70202..81641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAK14_RS00315 (RAK14_00315) - 70999..72282 (+) 1284 WP_003815911.1 amidase -
  RAK14_RS00320 (RAK14_00320) leuA 72423..74129 (-) 1707 WP_003815908.1 2-isopropylmalate synthase -
  RAK14_RS00325 (RAK14_00325) - 74556..75203 (-) 648 WP_003815907.1 hypothetical protein -
  RAK14_RS00330 (RAK14_00330) rpoN/rpoN1 75202..76641 (+) 1440 WP_010927240.1 RNA polymerase factor sigma-54 Machinery gene
  RAK14_RS00335 (RAK14_00335) - 76734..77036 (+) 303 WP_003815905.1 bacterioferritin-associated ferredoxin -
  RAK14_RS00340 (RAK14_00340) - 77064..77942 (-) 879 WP_003815902.1 LysR substrate-binding domain-containing protein -
  RAK14_RS00345 (RAK14_00345) - 78050..78979 (+) 930 WP_033452836.1 DMT family transporter -
  RAK14_RS00350 (RAK14_00350) bfr 79318..79794 (+) 477 WP_003815898.1 bacterioferritin -
  RAK14_RS00355 (RAK14_00355) - 79876..80559 (-) 684 WP_003815897.1 hypothetical protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 52341.57 Da        Isoelectric Point: 4.8131

>NTDB_id=868144 RAK14_RS00330 WP_010927240.1 75202..76641(+) (rpoN/rpoN1) [Bordetella bronchiseptica strain XX35]
MQNIVPMTRPALELRPGQHLTLTPQLQQSIRLLQLSSLELEAELSQALAENPLLERDEDPAREEQADPEREASVEADEAP
DTELRLDEMPGSGGVLPDDDSDLPQAARPDTLREHLLGQLALTRAAPRDAALCGLLIDELDENGYLDSPLEEILTWLPAE
AQADLDELRAALALLQSFDPAGIGARDMAECLQLQLRQPDPARLPEAADAEVLACARRICAQHLPLLATGNVARVREAVQ
CDEAVVRAAHALILRLEPRPGRAWTVPAADYAIPDVIVRRTRQGWQAVLNSAVVPRLHVNAVYAQVLGGQRQGGNAGLQA
QLQQARWMIRNVEQRFDTILRVAQAIVAHQQAFFTQGPAAMRPLILKDIAGELDLHESTISRATTQKYMLTPFGTLELKR
FFGAGVSTDGGDATSATAVQAVIRQMVADEDRAKPLSDSQIMQRLADQGIVIARRTVAKYREALRIAPATLRKAHAAGR

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=868144 RAK14_RS00330 WP_010927240.1 75202..76641(+) (rpoN/rpoN1) [Bordetella bronchiseptica strain XX35]
ATGCAAAATATTGTACCTATGACTCGTCCCGCGCTAGAACTTCGTCCTGGTCAACATTTGACGTTGACCCCTCAACTGCA
ACAGTCGATCCGGCTGTTGCAGCTGTCGTCGCTGGAGCTGGAGGCCGAACTGTCGCAGGCGCTGGCCGAGAATCCGTTGC
TGGAACGCGACGAAGATCCGGCGCGCGAGGAGCAGGCCGACCCCGAGCGCGAGGCCTCGGTCGAGGCCGACGAGGCGCCG
GATACCGAACTGCGGCTGGACGAGATGCCCGGTTCGGGCGGCGTTCTTCCCGACGACGACAGCGACCTGCCGCAGGCGGC
GCGCCCGGATACCCTGCGCGAACACCTGCTGGGGCAGCTGGCGCTGACGCGCGCCGCGCCGCGCGATGCGGCGCTGTGCG
GTCTGCTGATCGACGAACTGGACGAGAACGGCTACCTGGATTCGCCCCTGGAAGAGATCCTGACCTGGCTGCCGGCCGAG
GCGCAGGCCGACCTGGACGAGCTGCGCGCGGCGCTGGCATTGCTGCAGTCGTTCGATCCGGCAGGCATCGGCGCGCGCGA
CATGGCCGAATGCCTGCAGCTGCAGTTGCGCCAGCCCGATCCGGCGCGCCTGCCCGAGGCGGCCGATGCCGAGGTGCTGG
CCTGTGCGCGGCGCATCTGCGCGCAGCATCTGCCCTTGCTGGCCACCGGCAACGTGGCCCGTGTGCGCGAGGCCGTGCAA
TGCGACGAGGCGGTCGTGCGCGCCGCGCATGCGCTGATCCTGCGCCTGGAACCGCGGCCGGGCCGCGCCTGGACGGTGCC
GGCGGCCGATTACGCCATTCCCGACGTGATCGTGCGCCGTACCCGCCAGGGCTGGCAGGCGGTGCTCAACAGCGCCGTCG
TGCCGCGGCTGCATGTCAATGCCGTCTATGCGCAAGTGCTGGGCGGCCAGCGCCAGGGCGGCAACGCCGGCCTGCAGGCG
CAGCTGCAACAGGCGCGCTGGATGATACGCAACGTGGAGCAGCGCTTCGACACCATCCTGCGCGTGGCGCAGGCGATCGT
GGCGCATCAGCAGGCGTTCTTCACCCAGGGGCCGGCGGCCATGCGGCCGCTGATCCTCAAGGACATCGCCGGCGAACTGG
ATCTGCACGAATCGACCATTTCGCGCGCGACGACGCAGAAGTACATGCTCACACCGTTCGGTACGCTGGAGCTCAAGCGC
TTCTTTGGCGCGGGCGTGTCCACCGATGGCGGAGACGCCACCTCGGCCACCGCGGTGCAGGCGGTGATCCGCCAGATGGT
GGCCGACGAGGATCGCGCCAAGCCCTTGTCGGACAGCCAGATCATGCAGCGCCTGGCCGACCAGGGCATCGTCATTGCCC
GCCGCACGGTGGCCAAGTACCGCGAGGCGCTGCGGATTGCGCCAGCGACGCTGCGCAAGGCCCACGCCGCGGGTCGTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(106-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-473)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3LRZ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.419

100

0.511