Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NGH78_RS16125 Genome accession   NZ_AP024620
Coordinates   3285530..3286897 (-) Length   455 a.a.
NCBI ID   WP_161955024.1    Uniprot ID   -
Organism   Moorella sp. Hama-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3280530..3291897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGH78_RS16100 (hamaS1_32390) cysE 3281240..3281965 (-) 726 WP_109206910.1 serine O-acetyltransferase -
  NGH78_RS16105 (hamaS1_32400) ispF 3282551..3283045 (-) 495 WP_201261734.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  NGH78_RS16110 (hamaS1_32410) ispD 3283042..3283731 (-) 690 WP_109206908.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NGH78_RS16115 (hamaS1_32420) - 3283719..3284867 (-) 1149 WP_109206907.1 PIN/TRAM domain-containing protein -
  NGH78_RS16120 (hamaS1_32430) - 3284949..3285425 (-) 477 WP_109206906.1 CarD family transcriptional regulator -
  NGH78_RS16125 (hamaS1_32440) radA 3285530..3286897 (-) 1368 WP_161955024.1 DNA repair protein RadA Machinery gene
  NGH78_RS16130 (hamaS1_32450) - 3287521..3288267 (-) 747 WP_109206904.1 phosphatase -
  NGH78_RS16135 (hamaS1_32460) - 3289092..3289661 (-) 570 WP_109206903.1 HD-GYP domain-containing protein -
  NGH78_RS16140 (hamaS1_32480) - 3290248..3291693 (-) 1446 WP_109206901.1 ATP-binding protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 47859.31 Da        Isoelectric Point: 6.8293

>NTDB_id=86730 NGH78_RS16125 WP_161955024.1 3285530..3286897(-) (radA) [Moorella sp. Hama-1]
MAKLKERYVCQQCGYESLGFLGRCPGCGSWNSLVVEPGIPGGPQSSAAPASTPVLLAEVNDISAGRLVTSLGEWDRVLGG
GLVPGSLVLVGGAPGIGKSTLLLQVAHLLACKYGSILYVTGEESAGQTRLRAQRLGALDGAIYLLAETNIAAIIEQIEKL
QPVAVIIDSIQTMILPEVQAAPGSVSQVREGAARFLRLAKGGGPAVLLVGHVTKEGFLAGPRVLEHLVDCVLYLEGERYQ
AYRILRAVKNRFGSTNEIGVFEMAGSGLEEVRNPSAMLLAERPAGVAGSSVVACLEGTRPLLLEIQALVSRTNFGNPRRL
ATGIDFNRALLLAAVLEKRANLPLGGYDIYLNVAGGIAINEPAADLAVCLAIASSLQDRPLESRTLVLGEVGLAGEVRAV
AQLERRVEEAARLGFNRFIVPAGNSKGLKDRSGYEVYDVHTVGEALGLALVNTRG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=86730 NGH78_RS16125 WP_161955024.1 3285530..3286897(-) (radA) [Moorella sp. Hama-1]
CTGGCCAAGCTCAAGGAACGTTATGTCTGCCAGCAGTGCGGTTATGAATCCCTGGGCTTCCTGGGGCGCTGTCCCGGTTG
CGGCAGCTGGAACAGCCTGGTGGTAGAACCAGGAATACCCGGGGGTCCCCAAAGCTCCGCTGCACCGGCCTCCACTCCGG
TCCTGCTGGCTGAAGTTAACGATATCAGCGCCGGGAGGCTGGTAACTTCCCTGGGCGAGTGGGACCGGGTCCTGGGCGGC
GGCCTGGTACCAGGTTCTCTGGTCCTGGTAGGCGGGGCCCCCGGAATCGGCAAATCCACCCTGCTCCTGCAGGTCGCTCA
CCTGCTGGCCTGCAAGTATGGTAGTATCCTCTATGTCACCGGGGAGGAATCCGCCGGGCAGACCCGCCTCCGCGCCCAGC
GCCTGGGAGCGCTGGACGGCGCCATCTACCTGCTGGCTGAGACAAATATCGCGGCCATCATCGAGCAAATCGAAAAACTG
CAACCGGTAGCAGTTATTATTGATTCCATCCAGACCATGATCCTCCCTGAGGTCCAGGCCGCCCCGGGCAGTGTTTCCCA
GGTGCGGGAAGGAGCGGCCCGTTTTTTACGCCTGGCCAAGGGTGGCGGCCCGGCGGTATTGCTGGTGGGCCACGTTACCA
AGGAGGGCTTCCTGGCCGGTCCCCGGGTCCTGGAGCATCTGGTGGACTGTGTCCTCTATCTGGAGGGCGAACGTTACCAG
GCCTACCGCATCCTCCGGGCCGTCAAAAACCGTTTCGGCTCCACCAACGAGATTGGGGTTTTCGAAATGGCCGGTTCCGG
CCTGGAAGAAGTTCGTAATCCCTCGGCCATGCTCCTGGCCGAGAGGCCGGCCGGGGTGGCCGGTTCCAGCGTGGTGGCCT
GCCTGGAAGGGACCAGGCCCCTGCTGCTGGAGATCCAGGCCCTGGTGAGCCGGACCAACTTTGGTAATCCCCGCCGGCTG
GCAACGGGGATAGATTTCAACCGGGCCCTGTTACTGGCGGCTGTGCTGGAAAAGCGGGCCAACCTGCCCCTGGGGGGTTA
TGATATCTACCTCAACGTCGCCGGGGGCATTGCCATCAACGAACCGGCGGCGGACCTGGCCGTGTGTCTGGCCATAGCCT
CGAGCCTCCAGGACCGCCCCCTGGAATCCCGGACCCTGGTCCTGGGGGAGGTCGGCCTGGCGGGGGAAGTCCGGGCCGTA
GCGCAACTGGAACGGCGGGTTGAAGAGGCCGCCCGGCTGGGTTTTAACCGTTTTATCGTCCCGGCGGGCAATAGCAAGGG
CCTGAAAGATCGGTCCGGTTACGAAGTATATGATGTGCATACAGTAGGTGAAGCCCTGGGACTGGCTCTAGTCAATACCC
GGGGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(75-216)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)

Subunit ChlI of Mg-chelatase (ChlI)

(349-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

54.267

100

0.545
  radA Streptococcus mitis NCTC 12261

54.788

98.681

0.541
  radA Streptococcus pneumoniae Rx1

54.788

98.681

0.541
  radA Streptococcus pneumoniae D39

54.788

98.681

0.541
  radA Streptococcus pneumoniae R6

54.788

98.681

0.541
  radA Streptococcus pneumoniae TIGR4

54.788

98.681

0.541
  radA Streptococcus mitis SK321

54.788

98.681

0.541

Multiple sequence alignment