Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EUCAG14_RS04270 Genome accession   NZ_CP132137
Coordinates   975263..976624 (+) Length   453 a.a.
NCBI ID   WP_073381891.1    Uniprot ID   A0AB74EW29
Organism   Eubacterium callanderi strain DSM 107592     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 970263..981624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUCAG14_RS04245 (EUCAG14_08550) - 970402..971751 (+) 1350 WP_073381902.1 uroporphyrinogen decarboxylase family protein -
  EUCAG14_RS04250 (EUCAG14_08560) - 971810..972730 (+) 921 WP_083591059.1 GTP-binding protein -
  EUCAG14_RS04255 (EUCAG14_08570) - 972740..973381 (+) 642 WP_073381897.1 acetolactate decarboxylase -
  EUCAG14_RS04260 (EUCAG14_08580) - 973649..974698 (+) 1050 WP_073381894.1 PTS transporter subunit IIC -
  EUCAG14_RS04265 (EUCAG14_08590) - 974767..975252 (+) 486 WP_013381922.1 VanZ family protein -
  EUCAG14_RS04270 (EUCAG14_08600) radA 975263..976624 (+) 1362 WP_073381891.1 DNA repair protein RadA Machinery gene
  EUCAG14_RS04280 (EUCAG14_08610) ruvA 977027..977638 (+) 612 WP_013381920.1 Holliday junction branch migration protein RuvA -
  EUCAG14_RS04285 (EUCAG14_08620) ruvB 977650..978678 (+) 1029 WP_013381919.1 Holliday junction branch migration DNA helicase RuvB -
  EUCAG14_RS04290 (EUCAG14_08630) queA 978689..979723 (+) 1035 WP_073381889.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  EUCAG14_RS04295 (EUCAG14_08640) tgt 979736..980845 (+) 1110 WP_013381917.1 tRNA guanosine(34) transglycosylase Tgt -
  EUCAG14_RS04300 (EUCAG14_08650) yajC 980864..981307 (+) 444 WP_073381886.1 preprotein translocase subunit YajC -
  EUCAG14_RS04305 (EUCAG14_08660) scfA 981333..981473 (+) 141 WP_013381915.1 six-cysteine ranthipeptide SCIFF -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50506.88 Da        Isoelectric Point: 8.3489

>NTDB_id=867212 EUCAG14_RS04270 WP_073381891.1 975263..976624(+) (radA) [Eubacterium callanderi strain DSM 107592]
MAKIKKKFVCQNCGYSTPKWMGRCTECGEWNSFVEELDMPTEQGKKNTLERAVYTKPKRIEEIIPQKEDRFKTKNKELDR
VLGGGIVPGGVILLGGDPGIGKSTILLQTTENLGSQGLKILYISGEESEQQLKMRAVRMKVKSQNIFFLSEINVPYIVDT
ILHEKPDLVIIDSIQTMYSPTITSAPGSVSQIRENANVLMQIAKKDNISMILVGHVTKEGNIAGPRVLEHMVDTVLYFEG
EKYHTYRILRGVKNRFGSTNEIGIFEMAQDGLHEVANPSEMMLSSRPKNTCGSVVVPCMEGTRPLLIELQGLVSQTSFGN
PRRMATGMDYNRMVLLLAIMEKRLGLQLQNLDAYINVVGGMKIDEPALDLAVVSVLYSSFRNFEIPEDMMIIGEVGLTGE
VRNIQHIEKRLKEGAKLGFKRCIMPKGNARGLENTGLELLPVDNISKALNILK

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=867212 EUCAG14_RS04270 WP_073381891.1 975263..976624(+) (radA) [Eubacterium callanderi strain DSM 107592]
ATGGCGAAAATAAAAAAGAAATTTGTGTGCCAGAACTGCGGGTACAGCACGCCTAAATGGATGGGGCGCTGTACAGAATG
TGGCGAATGGAACTCCTTTGTTGAGGAGCTGGATATGCCCACTGAGCAGGGAAAGAAGAATACCCTTGAACGGGCGGTGT
ACACAAAACCCAAGCGTATTGAAGAAATTATTCCTCAGAAAGAGGATCGTTTTAAAACGAAGAATAAGGAGCTCGACCGC
GTCCTCGGCGGCGGCATTGTGCCTGGAGGCGTTATCCTTCTAGGAGGTGACCCGGGAATTGGAAAGTCAACCATTTTGCT
GCAGACGACGGAAAATCTTGGAAGCCAGGGGCTTAAAATCCTGTATATTTCAGGTGAGGAGTCCGAGCAGCAGCTCAAAA
TGCGCGCAGTCCGGATGAAGGTGAAATCCCAGAATATTTTTTTTCTGTCTGAGATTAACGTGCCCTATATCGTAGATACT
ATTTTGCATGAAAAGCCCGATCTGGTCATTATTGACTCGATTCAGACAATGTACAGTCCTACAATTACCTCAGCGCCCGG
AAGCGTCAGCCAGATAAGGGAGAACGCTAACGTGTTGATGCAGATTGCCAAAAAAGACAATATTTCGATGATTCTGGTCG
GACATGTGACCAAGGAAGGCAATATTGCCGGTCCCCGTGTGTTAGAACATATGGTTGATACGGTTTTGTATTTTGAGGGT
GAAAAGTACCATACCTACCGGATACTCAGAGGGGTCAAAAACCGTTTTGGCTCAACCAATGAGATCGGTATTTTCGAAAT
GGCGCAGGATGGCCTGCACGAGGTGGCAAATCCCTCTGAAATGATGCTGTCGAGCCGGCCGAAAAATACCTGTGGCTCTG
TGGTAGTGCCCTGCATGGAGGGGACACGTCCGCTTTTAATCGAGCTTCAGGGGCTCGTATCACAGACAAGCTTCGGTAAC
CCAAGGCGTATGGCCACCGGAATGGACTATAACCGTATGGTGCTGCTCCTGGCCATTATGGAAAAAAGGCTAGGATTACA
GCTTCAAAATCTTGATGCGTATATCAATGTGGTCGGGGGTATGAAGATAGATGAGCCGGCTCTTGATCTGGCCGTTGTGT
CTGTGCTATACTCAAGCTTCAGGAATTTTGAAATTCCAGAGGATATGATGATTATCGGCGAGGTAGGCCTGACTGGTGAG
GTCAGAAATATACAGCATATTGAAAAGCGCCTTAAAGAAGGCGCGAAGCTTGGCTTTAAGCGCTGTATCATGCCAAAGGG
CAATGCCCGGGGCCTGGAAAATACAGGGCTGGAGCTTCTGCCAGTCGATAATATATCTAAGGCCTTAAATATATTGAAAT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(355-428)

AAA domain (AAA_25)

(62-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

55.752

99.779

0.556
  radA Streptococcus mitis NCTC 12261

52.546

95.364

0.501
  radA Streptococcus mitis SK321

52.546

95.364

0.501
  radA Streptococcus pneumoniae Rx1

52.546

95.364

0.501
  radA Streptococcus pneumoniae D39

52.546

95.364

0.501
  radA Streptococcus pneumoniae R6

52.546

95.364

0.501
  radA Streptococcus pneumoniae TIGR4

52.546

95.364

0.501