Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   Q8W18_RS00575 Genome accession   NZ_CP132058
Coordinates   106121..107500 (+) Length   459 a.a.
NCBI ID   WP_058015795.1    Uniprot ID   -
Organism   Bacillus pumilus strain F12-21     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101121..112500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q8W18_RS00555 (Q8W18_00555) - 101463..101927 (+) 465 WP_003217184.1 CtsR family transcriptional regulator -
  Q8W18_RS00560 (Q8W18_00560) - 101942..102499 (+) 558 WP_034665655.1 UvrB/UvrC motif-containing protein -
  Q8W18_RS00565 (Q8W18_00565) - 102504..103595 (+) 1092 WP_306011546.1 protein arginine kinase -
  Q8W18_RS00570 (Q8W18_00570) clpC 103592..106027 (+) 2436 WP_003216969.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  Q8W18_RS00575 (Q8W18_00575) radA 106121..107500 (+) 1380 WP_058015795.1 DNA repair protein RadA Machinery gene
  Q8W18_RS00580 (Q8W18_00580) disA 107503..108582 (+) 1080 WP_007496267.1 DNA integrity scanning diadenylate cyclase DisA -
  Q8W18_RS00585 (Q8W18_00585) - 108736..109836 (+) 1101 WP_058015822.1 PIN/TRAM domain-containing protein -
  Q8W18_RS00590 (Q8W18_00590) ispD 109850..110539 (+) 690 WP_061406507.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  Q8W18_RS00595 (Q8W18_00595) ispF 110543..111019 (+) 477 WP_058015797.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50052.60 Da        Isoelectric Point: 7.9305

>NTDB_id=867025 Q8W18_RS00575 WP_058015795.1 106121..107500(+) (radA) [Bacillus pumilus strain F12-21]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPVNRRSAFNHSVQTIQKPSPISAIETSDEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIKSTSLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=867025 Q8W18_RS00575 WP_058015795.1 106121..107500(+) (radA) [Bacillus pumilus strain F12-21]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGTAAAGAGCCGGTAAACCGTCGAAGCGCTTTTAATCATTCTGTCC
AAACCATTCAAAAACCTTCACCTATTTCTGCAATTGAAACATCAGATGAACCCCGAATCAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGCTCTCTTGTTCTTATTGGCGGAGATCCTGGGATCGGCAAGTCCACATT
ATTATTACAAGTATCAGCACAGCTCTCAGACAAAAATCAGAATGTATTATACATATCCGGTGAGGAGTCCATTAAACAAA
CGAAGTTAAGAGCGGACCGCCTCGGCATAAAAAGCACCTCTTTACATGTTTTGGCTGAAACCGATATGGAATATATAACG
TCTGCTATACAAGAGATGAAACCCGCTTTTGTCGTGGTGGATTCGATTCAAACTGTTTATCAAAGCGATATTACGTCAGC
TCCTGGGTCTGTATCTCAAGTGAGAGAATGTACAGCGCAGCTGATGAAGATTGCCAAGACAAATGGGATTCCAATTTTTA
TTGTTGGTCACGTGACCAAAGAAGGCTCGATCGCAGGTCCACGTCTTTTAGAGCATATGGTGGACACGGTTCTTTATTTT
GAAGGTGAGCGTCATCATACGTTTCGTATCTTACGTGCAGTGAAAAACCGATTTGGTTCAACGAATGAACTAGGGATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAACCCATCCGAAATTTTCTTAGAAGAACGTTCGGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCTGTTCTTGTCGAGATACAAGCGTTAATTTCACCAACAAGCTTC
GGAAATCCGCGTAGAATGGCCACTGGTCTTGATCATAATCGAGTGTCACTGCTCATGGCGGTTTTAGAAAAACGTGTTGG
ACTGCTGCTGCAAAACCAGGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCAGCAATTGACTTGGCCA
TTGCGGTCAGTATTGCATCAAGCTTTAAAGACGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGTGTGCAGGAAGCTGCGAAACTAGGATTTAAGCGAATGTTTATTCC
TCAAGCGAATATAGATGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTCGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

86.9

99.782

0.867
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608