Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Q4S45_RS22970 Genome accession   NZ_CP131935
Coordinates   4983546..4985042 (+) Length   498 a.a.
NCBI ID   WP_305507911.1    Uniprot ID   A0AAT9WFR0
Organism   Massilia sp. R2A-15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4978546..4990042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q4S45_RS22945 (Q4S45_22945) - 4980048..4981046 (+) 999 WP_305507903.1 SIS domain-containing protein -
  Q4S45_RS22950 (Q4S45_22950) - 4981046..4981582 (+) 537 WP_305507905.1 HAD family hydrolase -
  Q4S45_RS22955 (Q4S45_22955) lptC 4981585..4982187 (+) 603 WP_305507907.1 LPS export ABC transporter periplasmic protein LptC -
  Q4S45_RS22960 (Q4S45_22960) lptA 4982221..4982787 (+) 567 WP_305507909.1 lipopolysaccharide transport periplasmic protein LptA -
  Q4S45_RS22965 (Q4S45_22965) lptB 4982784..4983542 (+) 759 WP_374046068.1 LPS export ABC transporter ATP-binding protein -
  Q4S45_RS22970 (Q4S45_22970) rpoN/rpoN1 4983546..4985042 (+) 1497 WP_305507911.1 RNA polymerase factor sigma-54 Machinery gene
  Q4S45_RS22975 (Q4S45_22975) hpf 4985112..4985465 (+) 354 WP_305507913.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  Q4S45_RS22980 (Q4S45_22980) - 4985558..4986640 (+) 1083 WP_305507915.1 enoyl-CoA hydratase/isomerase family protein -
  Q4S45_RS22985 (Q4S45_22985) rapZ 4986637..4987500 (-) 864 WP_305507917.1 RNase adapter RapZ -
  Q4S45_RS22990 (Q4S45_22990) - 4987604..4988068 (-) 465 WP_305507919.1 Ivy family c-type lysozyme inhibitor -
  Q4S45_RS22995 (Q4S45_22995) - 4988241..4989377 (+) 1137 WP_305507920.1 IS481 family transposase -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54559.99 Da        Isoelectric Point: 4.9103

>NTDB_id=866388 Q4S45_RS22970 WP_305507911.1 4983546..4985042(+) (rpoN/rpoN1) [Massilia sp. R2A-15]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELTQLLSDNPLLERLDDPLDNSVRLLADGAIGAPATPGEAAPAPG
AQDAPPGEGSGDAPAAGEGEGGGEGESPEAAGGDSEDWGEAGRNKTPDDEDSRPQIEASATTLRDHLMEQMRLAMLDTRD
RALVELMIDALDDNGYLTETLEEILERLPPELEIDIDELKTALSLLQSFDPVGVGARNASECLALQIRRMPHVPLVTRRM
ALDIVCKHLAWFAQRDFNKLKKALDCDDEDLREAQAVIRQCNPHPGAAFGSGASDYVVPDVIVRRSKNGWTVSLNQDVMP
RLRVNSMYANLLKNGKGEGQLGAQLQEAKWLIKNMRQRFDTILRVAEAIVERQRNFFSHGAVAMRPLVLREIADTLGLHE
STISRVTTQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIADMLGEQGMVIARRTV
AKYREALKIPPVALRKSL

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=866388 Q4S45_RS22970 WP_305507911.1 4983546..4985042(+) (rpoN/rpoN1) [Massilia sp. R2A-15]
ATGAAACAGTCACTGCAACTACGGACTTCCCAACACCTCGCGCTCACGCCGCAGCTGCAGCAGTCGATCCGTCTGCTGCA
GTTGTCCACGCTCGAGCTGCACCAGGAACTGACCCAGCTGCTGTCCGATAATCCGCTGCTCGAACGGCTCGACGACCCGC
TCGACAACTCGGTGCGCCTGCTGGCCGATGGCGCAATCGGCGCTCCCGCCACGCCGGGCGAGGCCGCGCCGGCCCCGGGC
GCGCAGGACGCGCCGCCAGGCGAGGGCAGCGGCGACGCGCCCGCCGCCGGTGAAGGCGAGGGCGGCGGCGAGGGAGAATC
CCCGGAGGCGGCCGGCGGCGACAGCGAGGACTGGGGCGAGGCGGGCCGCAACAAGACGCCCGACGACGAGGATTCGCGCC
CGCAGATCGAAGCGTCGGCCACCACCTTGCGCGACCATTTGATGGAGCAGATGCGCCTGGCGATGCTCGACACGCGCGAC
CGCGCGCTGGTCGAACTGATGATCGACGCGCTCGACGACAACGGCTACCTGACCGAAACGCTCGAAGAGATCCTCGAGCG
CCTGCCGCCCGAGCTCGAGATCGACATCGACGAACTCAAGACCGCGCTGTCGCTGCTGCAAAGCTTCGACCCGGTCGGCG
TGGGCGCCCGCAACGCGTCCGAATGCCTGGCGCTGCAGATCCGCCGCATGCCCCACGTGCCGCTGGTGACGCGGCGCATG
GCGCTCGACATCGTCTGCAAGCACCTCGCGTGGTTCGCCCAGCGCGACTTCAACAAGCTGAAAAAAGCCCTCGACTGCGA
CGACGAGGACCTGCGCGAGGCGCAGGCCGTGATTCGCCAGTGCAATCCGCATCCGGGCGCCGCGTTCGGCTCCGGCGCCT
CCGACTACGTGGTGCCGGACGTGATCGTGCGGCGCTCGAAGAACGGCTGGACCGTGTCGCTGAACCAGGATGTGATGCCG
CGCCTTCGCGTCAACAGCATGTACGCCAACCTGCTCAAGAACGGCAAGGGCGAGGGCCAGCTGGGCGCCCAGCTGCAGGA
GGCGAAGTGGCTGATCAAGAATATGCGCCAGCGCTTCGACACGATCTTGCGCGTGGCCGAGGCGATTGTTGAGCGGCAAC
GGAACTTTTTCTCGCACGGCGCGGTTGCCATGCGTCCACTTGTGCTGCGCGAAATTGCTGATACACTGGGACTACACGAG
AGTACGATATCTCGTGTGACAACCCAGAAATACATGCTTACACCGCATGGTATGTTTGAGTTGAAATACTTTTTTGGGAG
CCACGTCGCCACCGAAGCAGGAGGGGAAGCATCCTCCACGGCGATACGGGCACTCATCGTGCAACTCACAGGAGCCGAAG
ACCCTAAAAACCCTTTATCAGACAGTAAGATCGCAGACATGCTGGGGGAACAGGGGATGGTCATTGCACGACGCACGGTG
GCCAAATACCGTGAAGCGCTCAAGATCCCGCCCGTCGCCCTCCGCAAAAGTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(343-496)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

59.369

100

0.604