Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WHO52_RS01930 Genome accession   NZ_CP148132
Coordinates   417511..418188 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli isolate FELIX_MS75     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412511..423188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO52_RS01915 chiA 413878..416571 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  WHO52_RS01920 bfd 416740..416934 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WHO52_RS01925 bfr 417006..417482 (+) 477 WP_000675504.1 bacterioferritin -
  WHO52_RS01930 pilD 417511..418188 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  WHO52_RS01935 gspM 418188..418649 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  WHO52_RS01940 gspL 418646..419809 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  WHO52_RS01945 gspK 419824..420807 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  WHO52_RS01950 gspJ 420800..421387 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  WHO52_RS01955 gspI 421380..421757 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  WHO52_RS01960 gspH 421754..422263 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  WHO52_RS01965 gspG 422271..422708 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=865901 WHO52_RS01930 WP_000178154.1 417511..418188(-) (pilD) [Escherichia coli isolate FELIX_MS75]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=865901 WHO52_RS01930 WP_000178154.1 417511..418188(-) (pilD) [Escherichia coli isolate FELIX_MS75]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378