Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WHO18_RS13960 Genome accession   NZ_CP148128
Coordinates   2651974..2652384 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis isolate FELIX_MS620     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2646974..2657384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO18_RS13930 - 2647061..2647201 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  WHO18_RS13935 yqeF 2647507..2648238 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  WHO18_RS13940 cwlH 2648490..2649242 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  WHO18_RS13945 yqeD 2649429..2650055 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  WHO18_RS13950 gnd 2650074..2650967 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  WHO18_RS13955 yqeB 2651219..2651941 (+) 723 WP_010886572.1 hypothetical protein -
  WHO18_RS13960 nucA/comI 2651974..2652384 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  WHO18_RS13965 - 2652580..2652927 (+) 348 Protein_2704 sigma-70 family RNA polymerase sigma factor -
  WHO18_RS13970 spoIVCA 2652958..2654418 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  WHO18_RS13975 - 2654376..2654554 (-) 179 Protein_2706 hypothetical protein -
  WHO18_RS13980 arsC 2654909..2655328 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  WHO18_RS13985 acr3 2655340..2656380 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  WHO18_RS13990 yqcK 2656403..2656843 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  WHO18_RS13995 arsR 2656904..2657221 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=865841 WHO18_RS13960 WP_009967785.1 2651974..2652384(-) (nucA/comI) [Bacillus subtilis isolate FELIX_MS620]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=865841 WHO18_RS13960 WP_009967785.1 2651974..2652384(-) (nucA/comI) [Bacillus subtilis isolate FELIX_MS620]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529