Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   Q7574_RS15640 Genome accession   NZ_CP131867
Coordinates   3274191..3274991 (-) Length   266 a.a.
NCBI ID   WP_342063346.1    Uniprot ID   -
Organism   Acinetobacter pittii strain 2012N08-034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3269191..3279991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7574_RS15620 (Q7574_15680) - 3270256..3270954 (-) 699 WP_017398816.1 tetratricopeptide repeat protein -
  Q7574_RS15625 (Q7574_15685) hisS 3270983..3272275 (-) 1293 WP_032005186.1 histidine--tRNA ligase -
  Q7574_RS15630 (Q7574_15690) ispG 3272272..3273387 (-) 1116 WP_033849427.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  Q7574_RS15635 (Q7574_15695) - 3273409..3274200 (-) 792 WP_017385735.1 RodZ domain-containing protein -
  Q7574_RS15640 (Q7574_15700) pilF 3274191..3274991 (-) 801 WP_342063346.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  Q7574_RS15645 (Q7574_15705) rlmN 3275006..3276241 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  Q7574_RS15650 (Q7574_15710) ndk 3276365..3276796 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  Q7574_RS15655 (Q7574_15715) iscX 3276910..3277110 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  Q7574_RS15660 (Q7574_15720) - 3277210..3277761 (-) 552 WP_342063347.1 phosphatase PAP2 family protein -
  Q7574_RS15665 (Q7574_15725) - 3277786..3279075 (-) 1290 WP_081035543.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30102.32 Da        Isoelectric Point: 9.0417

>NTDB_id=865638 Q7574_RS15640 WP_342063346.1 3274191..3274991(-) (pilF) [Acinetobacter pittii strain 2012N08-034]
MSIPKLKTVLCMGISVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=865638 Q7574_RS15640 WP_342063346.1 3274191..3274991(-) (pilF) [Acinetobacter pittii strain 2012N08-034]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTTCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTCAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744