Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WHO22_RS13955 Genome accession   NZ_CP148122
Coordinates   2651899..2652309 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis isolate FELIX_MS513     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2646899..2657309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO22_RS13925 - 2646986..2647126 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  WHO22_RS13930 yqeF 2647432..2648163 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  WHO22_RS13935 cwlH 2648415..2649167 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  WHO22_RS13940 yqeD 2649354..2649980 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  WHO22_RS13945 gnd 2649999..2650892 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  WHO22_RS13950 yqeB 2651144..2651866 (+) 723 WP_010886572.1 hypothetical protein -
  WHO22_RS13955 nucA/comI 2651899..2652309 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  WHO22_RS13960 - 2652505..2652852 (+) 348 Protein_2703 sigma-70 family RNA polymerase sigma factor -
  WHO22_RS13965 spoIVCA 2652883..2654343 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  WHO22_RS13970 - 2654301..2654479 (-) 179 Protein_2705 hypothetical protein -
  WHO22_RS13975 arsC 2654834..2655253 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  WHO22_RS13980 acr3 2655265..2656305 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  WHO22_RS13985 yqcK 2656328..2656768 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  WHO22_RS13990 arsR 2656829..2657146 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=865443 WHO22_RS13955 WP_009967785.1 2651899..2652309(-) (nucA/comI) [Bacillus subtilis isolate FELIX_MS513]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=865443 WHO22_RS13955 WP_009967785.1 2651899..2652309(-) (nucA/comI) [Bacillus subtilis isolate FELIX_MS513]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529