Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WHO64_RS13935 Genome accession   NZ_CP148117
Coordinates   2651679..2652089 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis isolate FELIX_MS509     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2646679..2657089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO64_RS13905 - 2646766..2646906 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  WHO64_RS13910 yqeF 2647212..2647943 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  WHO64_RS13915 cwlH 2648195..2648947 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  WHO64_RS13920 yqeD 2649134..2649760 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  WHO64_RS13925 gnd 2649779..2650672 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  WHO64_RS13930 yqeB 2650924..2651646 (+) 723 WP_010886572.1 hypothetical protein -
  WHO64_RS13935 nucA/comI 2651679..2652089 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  WHO64_RS13940 - 2652285..2652632 (+) 348 Protein_2699 sigma-70 family RNA polymerase sigma factor -
  WHO64_RS13945 spoIVCA 2652663..2654123 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  WHO64_RS13950 - 2654081..2654259 (-) 179 Protein_2701 hypothetical protein -
  WHO64_RS13955 arsC 2654614..2655033 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  WHO64_RS13960 acr3 2655045..2656085 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  WHO64_RS13965 yqcK 2656108..2656548 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  WHO64_RS13970 arsR 2656609..2656926 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=865045 WHO64_RS13935 WP_009967785.1 2651679..2652089(-) (nucA/comI) [Bacillus subtilis isolate FELIX_MS509]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=865045 WHO64_RS13935 WP_009967785.1 2651679..2652089(-) (nucA/comI) [Bacillus subtilis isolate FELIX_MS509]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529