Detailed information NTG0864550

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: WHO32_RS01670
• Genome accession: NZ_CP148112
• Coordinates: 304030..305151 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis isolate FELIX_MS504
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 299030..310151

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHO32_RS01645	ycdA	299038..300102 (-)	1065	WP_003234750.1	DUF4352 domain-containing protein	-
WHO32_RS01650	-	300125..300256 (-)	132	WP_003246334.1	hypothetical protein	-
WHO32_RS01655	ycdB	300430..301848 (+)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
WHO32_RS01660	ycdC	302035..303396 (+)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
WHO32_RS01665	cwlK	303404..303907 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
WHO32_RS01670	rapF	304030..305151 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
WHO32_RS01675	ycdF	305258..306034 (+)	777	WP_003246415.1	SDR family oxidoreductase	-
WHO32_RS01680	ycdG	306059..307744 (+)	1686	WP_003234736.1	alpha-glucosidase	-
WHO32_RS01685	znuA	307932..308891 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
WHO32_RS01690	znuC	308947..309642 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=864550 WHO32_RS01670 WP_003246478.1 304030..305151(+) (rapF) [Bacillus subtilis isolate FELIX_MS504]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=864550 WHO32_RS01670 WP_003246478.1 304030..305151(+) (rapF) [Bacillus subtilis isolate FELIX_MS504]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405