Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WFE12_RS13955 Genome accession   NZ_CP147877
Coordinates   2653798..2654208 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2648798..2659208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WFE12_RS13925 - 2648885..2649025 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  WFE12_RS13930 yqeF 2649331..2650062 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  WFE12_RS13935 cwlH 2650314..2651066 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  WFE12_RS13940 yqeD 2651253..2651879 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  WFE12_RS13945 gnd 2651898..2652791 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  WFE12_RS13950 yqeB 2653043..2653765 (+) 723 WP_010886572.1 hypothetical protein -
  WFE12_RS13955 nucA/comI 2653798..2654208 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  WFE12_RS13960 - 2654404..2654751 (+) 348 Protein_2703 sigma-70 family RNA polymerase sigma factor -
  WFE12_RS13965 spoIVCA 2654782..2656242 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  WFE12_RS13970 - 2656200..2656378 (-) 179 Protein_2705 hypothetical protein -
  WFE12_RS13975 arsC 2656733..2657152 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  WFE12_RS13980 acr3 2657164..2658204 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  WFE12_RS13985 yqcK 2658227..2658667 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  WFE12_RS13990 arsR 2658728..2659045 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=863320 WFE12_RS13955 WP_009967785.1 2653798..2654208(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. 168]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=863320 WFE12_RS13955 WP_009967785.1 2653798..2654208(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. 168]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529