Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KI216_RS13505 Genome accession   NZ_AP024595
Coordinates   2873508..2874284 (-) Length   258 a.a.
NCBI ID   WP_018572782.1    Uniprot ID   -
Organism   Acinetobacter bouvetii strain JCM 18991     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2868508..2879284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI216_RS13485 (ACBO_26150) - 2869570..2870268 (-) 699 WP_005007485.1 YfgM family protein -
  KI216_RS13490 (ACBO_26160) hisS 2870299..2871594 (-) 1296 WP_005007484.1 histidine--tRNA ligase -
  KI216_RS13495 (ACBO_26170) ispG 2871591..2872706 (-) 1116 WP_005007482.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  KI216_RS13500 (ACBO_26180) - 2872729..2873517 (-) 789 WP_005007480.1 helix-turn-helix domain-containing protein -
  KI216_RS13505 (ACBO_26190) pilF 2873508..2874284 (-) 777 WP_018572782.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  KI216_RS13510 (ACBO_26200) rlmN 2874330..2875565 (-) 1236 WP_005007476.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  KI216_RS13515 (ACBO_26210) ndk 2875689..2876120 (-) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  KI216_RS13520 (ACBO_26220) iscX 2876231..2876431 (-) 201 WP_005007475.1 Fe-S cluster assembly protein IscX -
  KI216_RS13525 (ACBO_26230) - 2876519..2877070 (-) 552 WP_005007471.1 phosphatase PAP2 family protein -
  KI216_RS13530 (ACBO_26240) - 2877105..2878379 (-) 1275 WP_005007468.1 glycosyltransferase family 4 protein -
  KI216_RS13535 (ACBO_26250) - 2878615..2879025 (-) 411 WP_005007461.1 hypothetical protein -

Sequence

Protein


Download         Length: 258 a.a.        Molecular weight: 29021.96 Da        Isoelectric Point: 9.0430

>NTDB_id=86315 KI216_RS13505 WP_018572782.1 2873508..2874284(-) (pilF) [Acinetobacter bouvetii strain JCM 18991]
MTSFCLSAAVLTACQTPDSLGKKDPEKAAKVRTQLAVEYIKSGDLDAAKRALDQSLETNPRDAQANMMMGVLLQQEGSKL
NLEKADGYFRRAISLEPKNAQARNNYGTYLYQVGRYNEALEQLVIAGATLGYDQRSRSLENAGRIYLKLDDVANAEKTFK
KALQANRDSYVSMLELAEIFYLNQQTAAATQMYEQFVRAVGQQNQSARALWLGMRIARANGDKMGMQVLVNQLRALYPES
PEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 777 bp        

>NTDB_id=86315 KI216_RS13505 WP_018572782.1 2873508..2874284(-) (pilF) [Acinetobacter bouvetii strain JCM 18991]
ATGACATCTTTCTGCCTGTCGGCGGCAGTGCTGACTGCGTGTCAGACACCGGATTCGCTGGGAAAAAAAGATCCGGAAAA
AGCAGCCAAGGTGCGTACTCAATTGGCTGTAGAGTATATTAAATCTGGAGATTTGGACGCTGCTAAGCGTGCTCTGGATC
AATCCTTGGAAACGAATCCGCGGGATGCGCAAGCCAATATGATGATGGGCGTTTTATTGCAGCAGGAAGGCAGTAAGCTG
AATCTGGAGAAAGCCGATGGTTATTTCCGGCGCGCCATTTCTCTGGAGCCAAAAAATGCGCAAGCGCGTAACAACTATGG
TACGTATTTATATCAAGTTGGGCGCTATAATGAGGCGCTCGAGCAGTTGGTCATTGCTGGCGCCACATTAGGCTATGATC
AGCGCTCCAGATCGCTTGAAAACGCAGGCCGAATTTACTTGAAACTGGACGATGTTGCGAATGCTGAAAAAACATTCAAA
AAGGCTTTGCAAGCCAATCGTGATTCTTATGTTTCAATGCTAGAGTTGGCAGAAATTTTTTATTTGAACCAGCAAACCGC
TGCCGCAACGCAAATGTATGAACAGTTCGTACGTGCCGTGGGGCAGCAGAACCAAAGCGCTCGCGCGCTTTGGCTTGGTA
TGCGCATCGCACGTGCGAATGGGGACAAAATGGGGATGCAGGTGCTGGTAAACCAGCTTCGCGCGCTTTACCCGGAAAGC
CCTGAATACCAGCGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(89-121)

Tetratricopeptide repeat (TPR_16)

(34-73)

Tetratricopeptide repeat (TPR_8)

(137-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.357

100

0.764
  pilF Acinetobacter baylyi ADP1

70.866

98.45

0.698

Multiple sequence alignment