Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABJ42_RS02010 Genome accession   NZ_AP027479
Coordinates   445319..445996 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain LR-17-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440319..450996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ42_RS01995 (VEE14_03960) chiA 441686..444379 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  AABJ42_RS02000 (VEE14_03970) bfd 444548..444742 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABJ42_RS02005 (VEE14_03980) bfr 444814..445290 (+) 477 WP_000675504.1 bacterioferritin -
  AABJ42_RS02010 (VEE14_03990) pilD 445319..445996 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  AABJ42_RS02015 (VEE14_04000) gspM 445996..446457 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  AABJ42_RS02020 (VEE14_04010) gspL 446454..447617 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  AABJ42_RS02025 (VEE14_04020) gspK 447632..448615 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  AABJ42_RS02030 (VEE14_04030) gspJ 448608..449195 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  AABJ42_RS02035 (VEE14_04040) gspI 449188..449565 (-) 378 WP_097757311.1 type II secretion system minor pseudopilin GspI -
  AABJ42_RS02040 (VEE14_04050) gspH 449562..450071 (-) 510 WP_024238847.1 type II secretion system minor pseudopilin GspH -
  AABJ42_RS02045 (VEE14_04060) gspG 450079..450516 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=86276 AABJ42_RS02010 WP_000178161.1 445319..445996(-) (pilD) [Escherichia coli strain LR-17-1]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=86276 AABJ42_RS02010 WP_000178161.1 445319..445996(-) (pilD) [Escherichia coli strain LR-17-1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment