Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   Q5709_RS02130 Genome accession   NZ_CP131483
Coordinates   458688..459491 (+) Length   267 a.a.
NCBI ID   WP_005016505.1    Uniprot ID   A0A2T1J2S1
Organism   Acinetobacter radioresistens strain MC-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453688..464491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5709_RS02105 (Q5709_02105) - 454614..455891 (+) 1278 WP_005016524.1 glycosyltransferase family 4 protein -
  Q5709_RS02110 (Q5709_02110) - 455920..456471 (+) 552 WP_005016522.1 phosphatase PAP2 family protein -
  Q5709_RS02115 (Q5709_02115) iscX 456559..456759 (+) 201 WP_005016518.1 Fe-S cluster assembly protein IscX -
  Q5709_RS02120 (Q5709_02120) ndk 456874..457305 (+) 432 WP_005016515.1 nucleoside-diphosphate kinase -
  Q5709_RS02125 (Q5709_02125) rlmN 457428..458663 (+) 1236 WP_005016509.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  Q5709_RS02130 (Q5709_02130) pilF 458688..459491 (+) 804 WP_005016505.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  Q5709_RS02135 (Q5709_02135) - 459482..460285 (+) 804 WP_005016503.1 helix-turn-helix domain-containing protein -
  Q5709_RS02140 (Q5709_02140) ispG 460308..461423 (+) 1116 WP_005016500.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  Q5709_RS02145 (Q5709_02145) hisS 461420..462715 (+) 1296 WP_005016497.1 histidine--tRNA ligase -
  Q5709_RS02150 (Q5709_02150) - 462747..463451 (+) 705 WP_005016495.1 YfgM family protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30279.57 Da        Isoelectric Point: 9.5400

>NTDB_id=862632 Q5709_RS02130 WP_005016505.1 458688..459491(+) (pilF) [Acinetobacter radioresistens strain MC-14]
MRKPVYKTFVLLTVTLSALLVTACQTPDTLSKDPEKAVKVRTQLAAEYIKSGDLDAAKRALDQALEVDSRDATANMMMGV
LLQQEGSQLNLDKAESYFRRSISIEPKNAQARNNYGAYLYQIGRYKDAIEQLEIAGATLGYEQRYQALENLGRIYLKLGN
VANAEKTFKQALQANRDSSISMLELAEIFYLRQQIPAATQLYEQYVRRVGQKNQGARALWIGIRIARANDDKMGTQVLVN
QLRALFPDSQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=862632 Q5709_RS02130 WP_005016505.1 458688..459491(+) (pilF) [Acinetobacter radioresistens strain MC-14]
TTGAGAAAGCCGGTGTATAAAACATTTGTATTACTTACAGTGACTTTGTCGGCCTTGCTTGTTACTGCTTGCCAGACTCC
TGATACCCTCAGTAAGGATCCAGAAAAGGCAGTCAAAGTTCGAACCCAGCTGGCAGCGGAATATATTAAATCGGGTGATC
TGGATGCTGCTAAACGAGCACTTGACCAGGCACTTGAAGTTGATTCCAGAGACGCCACTGCGAACATGATGATGGGAGTA
CTGCTCCAGCAAGAGGGAAGTCAGCTAAATCTGGACAAGGCTGAGAGTTATTTCAGACGCTCTATTTCAATTGAGCCTAA
AAATGCACAGGCGCGTAATAATTATGGTGCCTATTTGTACCAGATCGGTCGTTATAAAGATGCGATCGAACAACTGGAAA
TTGCTGGTGCAACTTTAGGCTATGAGCAGCGTTACCAGGCGCTTGAAAATCTGGGACGAATTTATCTTAAGCTCGGCAAT
GTTGCGAATGCTGAAAAAACATTCAAACAGGCATTGCAAGCTAACCGCGATTCTTCCATTTCAATGTTAGAGTTAGCGGA
AATTTTCTATTTGCGGCAACAGATTCCTGCTGCTACACAGCTTTATGAACAATATGTGCGTCGTGTAGGGCAAAAGAATC
AGGGTGCGCGTGCTCTTTGGATCGGAATTCGAATTGCGCGTGCCAATGATGATAAAATGGGAACACAAGTTTTGGTGAAC
CAGTTACGTGCCTTGTTCCCGGACAGCCAAGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(44-82)

Tetratricopeptide repeat (TPR_16)

(150-208)

Tetratricopeptide repeat (TPR_17)

(98-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T1J2S1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.401

100

0.794
  pilF Acinetobacter baylyi ADP1

73.81

94.382

0.697