Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   Q4617_RS13040 Genome accession   NZ_CP131470
Coordinates   2663168..2663968 (-) Length   266 a.a.
NCBI ID   WP_004908991.1    Uniprot ID   -
Organism   Acinetobacter junii strain SC22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2658168..2668968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q4617_RS13020 (Q4617_13020) - 2659227..2659925 (-) 699 WP_004950316.1 tetratricopeptide repeat protein -
  Q4617_RS13025 (Q4617_13025) hisS 2659957..2661249 (-) 1293 WP_075696795.1 histidine--tRNA ligase -
  Q4617_RS13030 (Q4617_13030) ispG 2661246..2662361 (-) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  Q4617_RS13035 (Q4617_13035) - 2662386..2663177 (-) 792 WP_004950312.1 RodZ domain-containing protein -
  Q4617_RS13040 (Q4617_13040) pilF 2663168..2663968 (-) 801 WP_004908991.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  Q4617_RS13045 (Q4617_13045) rlmN 2663985..2665220 (-) 1236 WP_004950309.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  Q4617_RS13050 (Q4617_13050) ndk 2665342..2665773 (-) 432 WP_004908995.1 nucleoside-diphosphate kinase -
  Q4617_RS13055 (Q4617_13055) iscX 2665880..2666080 (-) 201 WP_004652532.1 Fe-S cluster assembly protein IscX -
  Q4617_RS13060 (Q4617_13060) - 2666163..2666714 (-) 552 WP_004908998.1 phosphatase PAP2 family protein -
  Q4617_RS13065 (Q4617_13065) - 2666742..2668058 (-) 1317 WP_171293790.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30366.44 Da        Isoelectric Point: 9.5600

>NTDB_id=862559 Q4617_RS13040 WP_004908991.1 2663168..2663968(-) (pilF) [Acinetobacter junii strain SC22]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=862559 Q4617_RS13040 WP_004908991.1 2663168..2663968(-) (pilF) [Acinetobacter junii strain SC22]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACTGCCTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTGG
ATTCAGCGAAACGCTCCCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAATATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGCAAACCAAACATGGAGAAAGCAGAAGCTTATTTTAAGCGAGCAATCTCTTCAGAACCCGATAA
TGCCCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAGGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_16)

(90-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.793
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68