Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KI226_RS02625 Genome accession   NZ_AP024590
Coordinates   541341..542774 (-) Length   477 a.a.
NCBI ID   WP_088221669.1    Uniprot ID   -
Organism   Enterobacter kobei strain JCM 8580     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 536341..547774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI226_RS02595 (ENKO_05100) elbB 537864..538517 (+) 654 WP_088221664.1 isoprenoid biosynthesis glyoxalase ElbB -
  KI226_RS02600 (ENKO_05110) mtgA 538514..539245 (+) 732 WP_088221665.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  KI226_RS02605 (ENKO_05120) npr 539288..539560 (-) 273 WP_088221666.1 PTS phosphocarrier protein NPr -
  KI226_RS02610 (ENKO_05130) rapZ 539557..540411 (-) 855 WP_072569561.1 RNase adapter RapZ -
  KI226_RS02615 (ENKO_05140) ptsN 540457..540948 (-) 492 WP_088221667.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KI226_RS02620 (ENKO_05150) hpf 541031..541318 (-) 288 WP_088221668.1 ribosome hibernation promoting factor -
  KI226_RS02625 (ENKO_05160) rpoN/rpoN1 541341..542774 (-) 1434 WP_088221669.1 RNA polymerase factor sigma-54 Machinery gene
  KI226_RS02630 (ENKO_05170) lptB 542822..543547 (-) 726 WP_088221670.1 LPS export ABC transporter ATP-binding protein -
  KI226_RS02635 (ENKO_05180) lptA 543554..544108 (-) 555 WP_072569566.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  KI226_RS02640 (ENKO_05190) lptC 544077..544652 (-) 576 WP_088221671.1 LPS export ABC transporter periplasmic protein LptC -
  KI226_RS02645 (ENKO_05200) kdsC 544649..545215 (-) 567 WP_088221672.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  KI226_RS02650 (ENKO_05210) kdsD 545231..546217 (-) 987 WP_088221673.1 arabinose-5-phosphate isomerase KdsD -
  KI226_RS02655 (ENKO_05220) - 546231..547208 (-) 978 WP_088221674.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53799.54 Da        Isoelectric Point: 4.3684

>NTDB_id=86243 KI226_RS02625 WP_088221669.1 541341..542774(-) (rpoN/rpoN1) [Enterobacter kobei strain JCM 8580]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEIDAQPAQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGNGVDYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVSLDD
ILESMGDEEVGLDEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFAKDTPWRDEAQLIISDHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHQGRWTVELNSDSIPRLQINQHYAAMCSGSRNDADSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFDKGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTDSGGEASSTAIRALVKKMIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=86243 KI226_RS02625 WP_088221669.1 541341..542774(-) (rpoN/rpoN1) [Enterobacter kobei strain JCM 8580]
ATGAAGCAAGGTTTGCAACTACGGCTCAGCCAACAACTGGCCATGACGCCACAATTGCAGCAGGCCATCCGCCTGCTGCA
ATTGTCTACGCTGGAATTGCAGCAAGAACTTCAGCAGGCGCTGGAAAGCAACCCCTTGCTGGAGCAAACCGATCTTCATG
AAGAGATAGACGCCCAGCCGGCTCAGGATACCGAAACCCTGGACTCTGTCGACGCCCTTGAACAGAAAGAGATGCCTGAC
GAGCTGCCACTGGATGCCAGCTGGGATGAGATCTATACCGCCGGCACGCCTTCCGGCAACGGTGTCGATTATCAGGACGA
TGAACTTCCGGTCTACCAGGGTGAAACCACCCAGTCCTTGCAGGATTACCTGATGTGGCAGGTGGAACTGACGCCGTTTT
CCGATACCGATCGCGCCATTGCCACCTCCATTGTTGATGCCGTAGATGACACCGGCTATCTGACCGTCTCGCTGGACGAT
ATTCTTGAAAGCATGGGCGACGAAGAGGTCGGGCTGGATGAAGTCGAGGCGGTACTCAAACGCGTCCAACGCTTTGATCC
GGTTGGCGTAGCCGCCCGCGATCTGCGCGACTGCCTGCTGATCCAGCTCTCCCAGTTTGCCAAAGACACCCCATGGCGCG
ACGAAGCGCAGTTGATCATCAGCGATCACCTCGATCTGCTGGCAAATCATGATTTCCGTTCACTGATGCGCGTGACGCGG
CTGAAAGAAGACGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCTCTCGATCCGCGCCCCGGTCAGTCGATCCAGACGGG
CGAACCCGAGTATGTGATCCCCGATGTGCTGGTGCGTAAGCATCAGGGGCGCTGGACGGTTGAGCTTAATTCCGACAGCA
TTCCCCGTCTGCAAATCAATCAGCACTACGCGGCTATGTGCAGCGGATCGCGCAATGACGCCGACAGTCAGTTTATCCGC
AGTAATTTGCAGGATGCCAAATGGCTGATCAAAAGCCTGGAAAGCCGTAACGACACGCTGCTGCGCGTCAGCCGCTGTAT
CGTCGAACAGCAGCAGGCCTTTTTTGATAAAGGCGAAGAATATATGAAACCGATGGTGCTGGCTGACATTGCCCAGGCCG
TCGAGATGCATGAATCGACCATTTCACGTGTTACAACCCAGAAGTACCTGCACAGCCCACGGGGCATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGACAGCGGCGGCGAAGCATCGTCCACCGCTATTCGCGCGCTGGTGAAGAAAAT
GATAGCCGCAGAAAACCCGGCGAAACCACTGAGTGACAGTAAGCTCACTACCCTGCTGTCCGACCAGGGTATCATGGTAG
CGCGCCGCACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.743

100

0.484

Multiple sequence alignment