Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   Q5794_RS23065 Genome accession   NZ_CP130958
Coordinates   4351053..4352105 (-) Length   350 a.a.
NCBI ID   WP_192995994.1    Uniprot ID   -
Organism   Priestia megaterium strain BS2-10     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4346053..4357105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5794_RS23025 (Q5794_22995) - 4347069..4347893 (+) 825 WP_116073644.1 YqhG family protein -
  Q5794_RS23030 (Q5794_23000) - 4347915..4348102 (-) 188 Protein_4481 YqzE family protein -
  Q5794_RS23035 (Q5794_23005) comGG 4348146..4348520 (-) 375 WP_013059171.1 competence type IV pilus minor pilin ComGG -
  Q5794_RS23040 (Q5794_23010) comGF 4348510..4348947 (-) 438 WP_013059172.1 competence type IV pilus minor pilin ComGF -
  Q5794_RS23045 (Q5794_23015) - 4348944..4349282 (-) 339 WP_013059173.1 type II secretion system protein -
  Q5794_RS23050 (Q5794_23020) comGD 4349266..4349748 (-) 483 WP_226897421.1 competence type IV pilus minor pilin ComGD -
  Q5794_RS23055 (Q5794_23025) comGC 4349690..4350001 (-) 312 WP_013059175.1 competence type IV pilus major pilin ComGC -
  Q5794_RS23060 (Q5794_23030) comGB 4350016..4351062 (-) 1047 WP_374988463.1 competence type IV pilus assembly protein ComGB -
  Q5794_RS23065 (Q5794_23035) comGA 4351053..4352105 (-) 1053 WP_192995994.1 competence type IV pilus ATPase ComGA Machinery gene
  Q5794_RS23070 (Q5794_23040) - 4352375..4352758 (-) 384 WP_374988464.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  Q5794_RS23075 (Q5794_23045) - 4353020..4353256 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  Q5794_RS23080 (Q5794_23050) - 4353294..4353926 (-) 633 WP_028410642.1 MBL fold metallo-hydrolase -
  Q5794_RS23085 (Q5794_23055) - 4354135..4354311 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  Q5794_RS23090 (Q5794_23060) - 4354347..4355249 (-) 903 WP_214984292.1 M14 family metallocarboxypeptidase -
  Q5794_RS23095 (Q5794_23065) - 4355361..4356329 (-) 969 WP_013059183.1 ROK family glucokinase -
  Q5794_RS23100 (Q5794_23070) - 4356332..4356538 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39552.96 Da        Isoelectric Point: 9.2325

>NTDB_id=862334 Q5794_RS23065 WP_192995994.1 4351053..4352105(-) (comGA) [Priestia megaterium strain BS2-10]
MTEIEQFATYIIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGGN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=862334 Q5794_RS23065 WP_192995994.1 4351053..4352105(-) (comGA) [Priestia megaterium strain BS2-10]
TTGACCGAAATTGAACAGTTTGCAACTTACATTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAGGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGGAAACCTCAAAATGGTGCATTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACACACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACACTAGAAGACCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTACA
GGTGAATGAACGGGCGGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGACATTATTATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAGTTTGGCGTTAGCTTTCAAGAAATGGAGCAGACTCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCCGCTTGTAAAAAAATGAGGA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGGAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586