Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   Q5794_RS16975 Genome accession   NZ_CP130958
Coordinates   3237813..3238235 (+) Length   140 a.a.
NCBI ID   WP_214985967.1    Uniprot ID   -
Organism   Priestia megaterium strain BS2-10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3232813..3243235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5794_RS16945 (Q5794_16920) - 3233001..3233630 (-) 630 WP_214985959.1 LysE family translocator -
  Q5794_RS16950 (Q5794_16925) - 3233658..3234491 (-) 834 WP_214985962.1 AraC family transcriptional regulator -
  Q5794_RS16955 (Q5794_16930) - 3234715..3235536 (+) 822 WP_374988178.1 sensor histidine kinase -
  Q5794_RS16960 (Q5794_16935) - 3235537..3236178 (+) 642 WP_053487482.1 response regulator transcription factor -
  Q5794_RS16965 (Q5794_16940) - 3236387..3237178 (+) 792 WP_214986176.1 PepSY domain-containing protein -
  Q5794_RS16970 (Q5794_16945) - 3237208..3237672 (+) 465 WP_214985965.1 FixH family protein -
  Q5794_RS16975 (Q5794_16950) nucA/comI 3237813..3238235 (+) 423 WP_214985967.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  Q5794_RS16980 (Q5794_16955) - 3238389..3238742 (+) 354 WP_223272376.1 DUF1516 family protein -
  Q5794_RS16985 (Q5794_16960) - 3238783..3239376 (-) 594 WP_075421022.1 hypothetical protein -
  Q5794_RS16990 (Q5794_16965) - 3239664..3240149 (-) 486 WP_214985972.1 hypothetical protein -
  Q5794_RS16995 (Q5794_16970) fsa 3240228..3240896 (-) 669 WP_129710569.1 fructose-6-phosphate aldolase -
  Q5794_RS17000 (Q5794_16975) gnd 3240946..3241839 (-) 894 WP_192994374.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15311.14 Da        Isoelectric Point: 4.4990

>NTDB_id=862321 Q5794_RS16975 WP_214985967.1 3237813..3238235(+) (nucA/comI) [Priestia megaterium strain BS2-10]
MKKIVSGILILVLAVIVIIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=862321 Q5794_RS16975 WP_214985967.1 3237813..3238235(+) (nucA/comI) [Priestia megaterium strain BS2-10]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCTGTTATTGTTATAATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGAGCTGAAGAAAACCGCCGTGAATCTTTAAAA
GGCATTCCGACTAAAAGCGGCTACGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGCGCCGATAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGGTCTTGGATTTCTCATCAAGTAGATGAGTTTCCAGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507