Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WCU17_RS24250 Genome accession   NZ_CP147618
Coordinates   5211479..5212972 (+) Length   497 a.a.
NCBI ID   WP_058342978.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain GN04844     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5206479..5217972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU17_RS24220 (WCU17_24220) - 5206692..5207501 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  WCU17_RS24225 (WCU17_24225) kdsD 5207914..5208894 (+) 981 WP_003134758.1 arabinose-5-phosphate isomerase KdsD -
  WCU17_RS24230 (WCU17_24230) - 5208894..5209433 (+) 540 WP_003094350.1 HAD family hydrolase -
  WCU17_RS24235 (WCU17_24235) lptC 5209442..5210014 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  WCU17_RS24240 (WCU17_24240) lptA 5210001..5210528 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  WCU17_RS24245 (WCU17_24245) lptB 5210528..5211253 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  WCU17_RS24250 (WCU17_24250) rpoN/rpoN1 5211479..5212972 (+) 1494 WP_058342978.1 RNA polymerase factor sigma-54 Machinery gene
  WCU17_RS24255 (WCU17_24255) raiA 5213050..5213358 (+) 309 WP_003094359.1 ribosome-associated translation inhibitor RaiA -
  WCU17_RS24260 (WCU17_24260) ptsN 5213372..5213836 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WCU17_RS24265 (WCU17_24265) rapZ 5213838..5214698 (+) 861 WP_003094361.1 RNase adapter RapZ -
  WCU17_RS24270 (WCU17_24270) - 5214728..5215000 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  WCU17_RS24275 (WCU17_24275) - 5215097..5216029 (-) 933 WP_003120918.1 ZIP family metal transporter -
  WCU17_RS24280 (WCU17_24280) - 5216048..5216659 (-) 612 WP_338851702.1 superoxide dismutase -
  WCU17_RS24285 (WCU17_24285) - 5216672..5217121 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56072.23 Da        Isoelectric Point: 4.4327

>NTDB_id=862058 WCU17_RS24250 WP_058342978.1 5211479..5212972(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain GN04844]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAATATQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=862058 WCU17_RS24250 WP_058342978.1 5211479..5212972(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain GN04844]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCACCGCGACCCAGGAGAGCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAGTGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGTTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCACGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477