Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WCU19_RS02055 Genome accession   NZ_CP147611
Coordinates   434726..435403 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain GN06758     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 429726..440403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU19_RS02040 (WCU19_02040) chiA 431084..433786 (+) 2703 WP_000773166.1 bifunctional chitinase/lysozyme -
  WCU19_RS02045 (WCU19_02045) bfd 433955..434149 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WCU19_RS02050 (WCU19_02050) bfr 434221..434697 (+) 477 WP_000675504.1 bacterioferritin -
  WCU19_RS02055 (WCU19_02055) pilD 434726..435403 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  WCU19_RS02060 (WCU19_02060) gspM 435403..435864 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  WCU19_RS02065 (WCU19_02065) gspL 435861..437024 (-) 1164 WP_001332436.1 type II secretion system protein GspL -
  WCU19_RS02070 (WCU19_02070) gspK 437039..438022 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  WCU19_RS02075 (WCU19_02075) gspJ 438015..438602 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  WCU19_RS02080 (WCU19_02080) gspI 438595..438990 (-) 396 WP_001041745.1 type II secretion system minor pseudopilin GspI -
  WCU19_RS02085 (WCU19_02085) gspH 438987..439496 (-) 510 WP_001076038.1 type II secretion system minor pseudopilin GspH -
  WCU19_RS02090 (WCU19_02090) gspG 439504..439941 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=861747 WCU19_RS02055 WP_000178175.1 434726..435403(-) (pilD) [Escherichia coli strain GN06758]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=861747 WCU19_RS02055 WP_000178175.1 434726..435403(-) (pilD) [Escherichia coli strain GN06758]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387