Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WCU38_RS02820 Genome accession   NZ_CP147578
Coordinates   552624..554057 (-) Length   477 a.a.
NCBI ID   WP_021524545.1    Uniprot ID   -
Organism   Escherichia coli strain GN05476     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 547624..559057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU38_RS02785 (WCU38_02785) elbB 548316..548969 (+) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -
  WCU38_RS02790 (WCU38_02790) mtgA 548966..549694 (+) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  WCU38_RS02795 (WCU38_02795) yrbL 549691..550323 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  WCU38_RS02800 (WCU38_02800) npr 550536..550808 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  WCU38_RS02805 (WCU38_02805) rapZ 550805..551659 (-) 855 WP_000243741.1 RNase adapter RapZ -
  WCU38_RS02810 (WCU38_02810) ptsN 551705..552196 (-) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WCU38_RS02815 (WCU38_02815) hpf 552314..552601 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  WCU38_RS02820 (WCU38_02820) rpoN/rpoN1 552624..554057 (-) 1434 WP_021524545.1 RNA polymerase factor sigma-54 Machinery gene
  WCU38_RS02825 (WCU38_02825) lptB 554105..554830 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  WCU38_RS02830 (WCU38_02830) lptA 554837..555394 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  WCU38_RS02835 (WCU38_02835) lptC 555363..555938 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  WCU38_RS02840 (WCU38_02840) kdsC 555935..556501 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WCU38_RS02845 (WCU38_02845) kdsD 556522..557508 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  WCU38_RS02850 (WCU38_02850) yrbG 557522..558499 (-) 978 WP_000922880.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53975.76 Da        Isoelectric Point: 4.3639

>NTDB_id=861387 WCU38_RS02820 WP_021524545.1 552624..554057(-) (rpoN/rpoN1) [Escherichia coli strain GN05476]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEIPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=861387 WCU38_RS02820 WP_021524545.1 552624..554057(-) (rpoN/rpoN1) [Escherichia coli strain GN05476]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATTCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTTTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCTGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGCGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465