Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WCU32_RS01945 Genome accession   NZ_CP147574
Coordinates   418729..419406 (-) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain GN03590     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413729..424406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU32_RS01930 (WCU32_01930) chiA 415087..417789 (+) 2703 WP_000773180.1 bifunctional chitinase/lysozyme -
  WCU32_RS01935 (WCU32_01935) bfd 417958..418152 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WCU32_RS01940 (WCU32_01940) bfr 418224..418700 (+) 477 WP_000675504.1 bacterioferritin -
  WCU32_RS01945 (WCU32_01945) pilD 418729..419406 (-) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  WCU32_RS01950 (WCU32_01950) gspM 419406..419867 (-) 462 WP_001296465.1 type II secretion system protein GspM -
  WCU32_RS01955 (WCU32_01955) gspL 419864..421027 (-) 1164 WP_001296464.1 type II secretion system protein GspL -
  WCU32_RS01960 (WCU32_01960) gspK 421042..422025 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  WCU32_RS01965 (WCU32_01965) gspJ 422018..422605 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  WCU32_RS01970 (WCU32_01970) gspI 422598..422975 (-) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  WCU32_RS01975 (WCU32_01975) gspH 422972..423481 (-) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  WCU32_RS01980 (WCU32_01980) gspG 423489..423926 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=861297 WCU32_RS01945 WP_000178170.1 418729..419406(-) (pilD) [Escherichia coli strain GN03590]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=861297 WCU32_RS01945 WP_000178170.1 418729..419406(-) (pilD) [Escherichia coli strain GN03590]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382