Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WCU23_RS02075 Genome accession   NZ_CP147571
Coordinates   439043..439720 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain GN04275     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 434043..444720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU23_RS02060 (WCU23_02060) chiA 435401..438103 (+) 2703 WP_000773166.1 bifunctional chitinase/lysozyme -
  WCU23_RS02065 (WCU23_02065) bfd 438272..438466 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WCU23_RS02070 (WCU23_02070) bfr 438538..439014 (+) 477 WP_000675504.1 bacterioferritin -
  WCU23_RS02075 (WCU23_02075) pilD 439043..439720 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  WCU23_RS02080 (WCU23_02080) gspM 439720..440181 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  WCU23_RS02085 (WCU23_02085) gspL 440178..441341 (-) 1164 WP_001332436.1 type II secretion system protein GspL -
  WCU23_RS02090 (WCU23_02090) gspK 441356..442339 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  WCU23_RS02095 (WCU23_02095) gspJ 442332..442919 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  WCU23_RS02100 (WCU23_02100) gspI 442912..443307 (-) 396 WP_001041745.1 type II secretion system minor pseudopilin GspI -
  WCU23_RS02105 (WCU23_02105) gspH 443304..443813 (-) 510 WP_001076038.1 type II secretion system minor pseudopilin GspH -
  WCU23_RS02110 (WCU23_02110) gspG 443821..444258 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=861124 WCU23_RS02075 WP_000178175.1 439043..439720(-) (pilD) [Escherichia coli strain GN04275]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=861124 WCU23_RS02075 WP_000178175.1 439043..439720(-) (pilD) [Escherichia coli strain GN04275]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387