Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QUE55_RS03730 Genome accession   NZ_AP027445
Coordinates   460294..460971 (-) Length   225 a.a.
NCBI ID   WP_025670758.1    Uniprot ID   -
Organism   Escherichia coli strain EC291 isolate EC291     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 455294..465971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE55_RS03710 (MUTS14_07170) - 455725..456422 (+) 698 WP_223367350.1 IS1 family transposase -
  QUE55_RS03715 (MUTS14_07180) chiA 456661..459354 (+) 2694 WP_025670759.1 bifunctional chitinase/lysozyme -
  QUE55_RS03720 (MUTS14_07190) bfd 459523..459717 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QUE55_RS03725 (MUTS14_07200) bfr 459789..460265 (+) 477 WP_000675504.1 bacterioferritin -
  QUE55_RS03730 (MUTS14_07210) pilD 460294..460971 (-) 678 WP_025670758.1 prepilin peptidase GspO Machinery gene
  QUE55_RS03735 (MUTS14_07220) gspM 460971..461432 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  QUE55_RS03740 (MUTS14_07230) gspL 461429..462592 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  QUE55_RS03745 (MUTS14_07240) gspK 462607..463590 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  QUE55_RS03750 (MUTS14_07250) gspJ 463583..464170 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  QUE55_RS03755 (MUTS14_07260) gspI 464163..464540 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  QUE55_RS03760 (MUTS14_07270) gspH 464537..465046 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  QUE55_RS03765 (MUTS14_07280) gspG 465054..465491 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24888.75 Da        Isoelectric Point: 7.9563

>NTDB_id=86033 QUE55_RS03730 WP_025670758.1 460294..460971(-) (pilD) [Escherichia coli strain EC291 isolate EC291]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLSDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=86033 QUE55_RS03730 WP_025670758.1 460294..460971(-) (pilD) [Escherichia coli strain EC291 isolate EC291]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGTCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment