Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Atep_RS03050 Genome accession   NZ_AP024563
Coordinates   650399..651436 (-) Length   345 a.a.
NCBI ID   WP_213380220.1    Uniprot ID   -
Organism   Allochromatium tepidum strain NZ     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 645399..656436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Atep_RS03035 (Atep_05980) - 646841..648076 (+) 1236 WP_213380217.1 PP2C family protein-serine/threonine phosphatase -
  Atep_RS03040 (Atep_05990) - 648130..649203 (+) 1074 WP_213380218.1 HD domain-containing phosphohydrolase -
  Atep_RS03045 (Atep_06000) pilU 649213..650367 (-) 1155 WP_213380219.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Atep_RS03050 (Atep_06010) pilT 650399..651436 (-) 1038 WP_213380220.1 type IV pilus twitching motility protein PilT Machinery gene
  Atep_RS03055 (Atep_06020) purE 651514..652029 (-) 516 WP_213380221.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  Atep_RS03060 (Atep_06030) purK 652026..653180 (-) 1155 WP_213380222.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  Atep_RS03065 (Atep_06040) hemF 653344..654264 (+) 921 WP_213380223.1 oxygen-dependent coproporphyrinogen oxidase -
  Atep_RS03070 (Atep_06050) - 654273..655349 (-) 1077 WP_213380224.1 glycosyltransferase -
  Atep_RS03075 (Atep_06060) - 655364..655747 (-) 384 WP_213380225.1 PIN domain nuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38314.08 Da        Isoelectric Point: 7.0820

>NTDB_id=86012 Atep_RS03050 WP_213380220.1 650399..651436(-) (pilT) [Allochromatium tepidum strain NZ]
MDIAELLAFSVKNNASDLHLSSGLPPMIRVDGDMRRINVPPLEHRAVHSLVYDIMNDKQREDYEEFLETDFSFEIPGVAR
FRVNAFNQKRGAAAVFRTIPSKVLTLEDIKAPKSFKDIIDVPRGLILVTGPTGSGKSTTLAAMIDHINDNKYEHILTIED
PIEFVHVSKKCLVNQREVHKDTLGFSEALRSALRQDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMVRAMLSESLRAVISQTLLKKTGGGRIAAHEIMIGTPAIRNLIREAKIAQMYSSIQTGQAHGMQTLDQNL
KELLGKGLIARQDARSKAQNKEDFN

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=86012 Atep_RS03050 WP_213380220.1 650399..651436(-) (pilT) [Allochromatium tepidum strain NZ]
GTGGACATCGCCGAACTGCTTGCCTTCAGCGTCAAGAACAACGCCTCTGACTTGCATCTGTCGTCCGGGCTGCCGCCCAT
GATCCGGGTCGACGGCGACATGCGCCGCATCAATGTTCCGCCCCTGGAACATCGCGCGGTCCATTCGCTCGTCTACGACA
TCATGAACGACAAGCAGCGCGAGGACTACGAGGAGTTCCTGGAGACCGACTTCTCGTTCGAGATCCCCGGCGTGGCGCGT
TTTCGCGTCAACGCCTTCAACCAGAAGCGCGGCGCGGCCGCCGTCTTCCGGACCATTCCTTCCAAGGTACTGACGCTCGA
GGACATCAAGGCCCCCAAGAGCTTCAAGGACATCATCGACGTCCCGCGCGGACTGATCCTGGTCACGGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCGGCCATGATCGACCACATCAACGACAACAAGTACGAACACATCCTCACCATCGAGGAT
CCGATCGAGTTCGTGCACGTCAGCAAGAAGTGTCTGGTCAACCAGCGCGAGGTGCACAAGGACACCCTCGGTTTCAGCGA
GGCCCTGCGCTCGGCCCTGCGCCAGGATCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAGACCATCCGGCTGG
CCCTGACCGCCGCCGAGACCGGCCATCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTCTTCCCGGCGGCCGAGAAGGACATGGTGCGCGCCATGCTCTCGGAGTCGCTGCGCGCGGTCATCTCGCAGAC
GCTGCTGAAGAAGACCGGCGGCGGCCGTATCGCCGCGCACGAGATCATGATCGGCACCCCGGCCATCCGCAACCTCATCC
GCGAGGCCAAGATCGCCCAGATGTACTCGTCGATCCAGACCGGCCAGGCGCACGGGATGCAAACCCTGGATCAGAATCTC
AAGGAGCTGCTGGGCAAGGGCCTCATCGCCCGCCAGGACGCTCGATCCAAGGCGCAGAACAAAGAGGATTTCAATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

81.395

99.71

0.812
  pilT Pseudomonas aeruginosa PAK

80.814

99.71

0.806
  pilT Acinetobacter nosocomialis M2

80.523

99.71

0.803
  pilT Acinetobacter baumannii D1279779

80.523

99.71

0.803
  pilT Acinetobacter baumannii strain A118

80.523

99.71

0.803
  pilT Acinetobacter baylyi ADP1

79.07

99.71

0.788
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Neisseria meningitidis 8013

68.786

100

0.69
  pilT Neisseria gonorrhoeae MS11

68.497

100

0.687
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.027

98.261

0.678
  pilT Vibrio cholerae strain A1552

69.027

98.261

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Pseudomonas stutzeri DSM 10701

40.87

100

0.409
  pilU Vibrio cholerae strain A1552

39.42

100

0.394
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment