Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WAZ81_RS19570 Genome accession   NZ_CP147105
Coordinates   4051850..4052317 (-) Length   155 a.a.
NCBI ID   WP_000340171.1    Uniprot ID   -
Organism   Escherichia coli strain KKa014     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4046850..4057317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ81_RS19545 (WAZ81_19565) rpsG 4046991..4047461 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  WAZ81_RS19550 (WAZ81_19570) fusA 4047558..4049672 (+) 2115 WP_000124700.1 elongation factor G -
  WAZ81_RS19555 (WAZ81_19575) tuf 4049743..4050927 (+) 1185 WP_000031783.1 elongation factor Tu -
  WAZ81_RS19560 (WAZ81_19580) bfd 4051110..4051304 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  WAZ81_RS19565 (WAZ81_19585) bfr 4051377..4051853 (+) 477 WP_000675515.1 bacterioferritin -
  WAZ81_RS19570 (WAZ81_19590) pilD 4051850..4052317 (-) 468 WP_000340171.1 A24 family peptidase Machinery gene
  WAZ81_RS19575 (WAZ81_19595) rpsJ 4052696..4053007 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  WAZ81_RS19580 (WAZ81_19600) rplC 4053040..4053669 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  WAZ81_RS19585 (WAZ81_19605) rplD 4053680..4054285 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  WAZ81_RS19590 (WAZ81_19610) rplW 4054282..4054584 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  WAZ81_RS19595 (WAZ81_19615) rplB 4054602..4055423 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  WAZ81_RS19600 (WAZ81_19620) rpsS 4055440..4055718 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  WAZ81_RS19605 (WAZ81_19625) rplV 4055733..4056065 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  WAZ81_RS19610 (WAZ81_19630) rpsC 4056083..4056784 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  WAZ81_RS19615 (WAZ81_19635) rplP 4056797..4057207 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16938.08 Da        Isoelectric Point: 8.1296

>NTDB_id=858996 WAZ81_RS19570 WP_000340171.1 4051850..4052317(-) (pilD) [Escherichia coli strain KKa014]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=858996 WAZ81_RS19570 WP_000340171.1 4051850..4052317(-) (pilD) [Escherichia coli strain KKa014]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361