Detailed information NTG0858858

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: WAZ92_RS00850
• Genome accession: NZ_CP147101
• Coordinates: 180289..181272 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain KFu021
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 175289..186272

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WAZ92_RS00835 (WAZ92_00835)	dppA	176432..178039 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
WAZ92_RS00840 (WAZ92_00840)	dppB	178347..179366 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
WAZ92_RS00845 (WAZ92_00845)	dppC	179376..180278 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
WAZ92_RS00850 (WAZ92_00850)	amiE	180289..181272 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
WAZ92_RS00855 (WAZ92_00855)	dppF	181269..182273 (+)	1005	WP_000107018.1	dipeptide ABC transporter ATP-binding subunit DppF	-
WAZ92_RS00860 (WAZ92_00860)	yhjV	182303..183574 (-)	1272	WP_001296805.1	aromatic amino acid transport family protein	-
WAZ92_RS00865 (WAZ92_00865)	-	184050..184157 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
WAZ92_RS00870 (WAZ92_00870)	-	184533..184640 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
WAZ92_RS00875 (WAZ92_00875)	-	185016..185123 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=858858 WAZ92_RS00850 WP_001196486.1 180289..181272(+) (amiE) [Escherichia coli strain KFu021]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=858858 WAZ92_RS00850 WP_001196486.1 180289..181272(+) (amiE) [Escherichia coli strain KFu021]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404