Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WAZ89_RS04150 Genome accession   NZ_CP147083
Coordinates   563625..565058 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain JSWP006     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 558625..570058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ89_RS04120 (WAZ89_04120) yrbG 559183..560160 (+) 978 WP_000922873.1 calcium/sodium antiporter -
  WAZ89_RS04125 (WAZ89_04125) kdsD 560174..561160 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  WAZ89_RS04130 (WAZ89_04130) kdsC 561181..561747 (+) 567 WP_000030006.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WAZ89_RS04135 (WAZ89_04135) lptC 561744..562319 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  WAZ89_RS04140 (WAZ89_04140) lptA 562288..562845 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  WAZ89_RS04145 (WAZ89_04145) lptB 562852..563577 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  WAZ89_RS04150 (WAZ89_04150) rpoN/rpoN1 563625..565058 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  WAZ89_RS04155 (WAZ89_04155) hpf 565081..565368 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  WAZ89_RS04160 (WAZ89_04160) ptsN 565486..565977 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WAZ89_RS04165 (WAZ89_04165) rapZ 566023..566877 (+) 855 WP_000243741.1 RNase adapter RapZ -
  WAZ89_RS04170 (WAZ89_04170) npr 566874..567146 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  WAZ89_RS04175 (WAZ89_04175) yrbL 567359..567991 (+) 633 WP_000620396.1 PhoP regulatory network protein YrbL -
  WAZ89_RS04180 (WAZ89_04180) mtgA 567988..568716 (-) 729 WP_000047064.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  WAZ89_RS04185 (WAZ89_04185) elbB 568713..569366 (-) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=858628 WAZ89_RS04150 WP_000809051.1 563625..565058(+) (rpoN/rpoN1) [Escherichia coli strain JSWP006]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=858628 WAZ89_RS04150 WP_000809051.1 563625..565058(+) (rpoN/rpoN1) [Escherichia coli strain JSWP006]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGATACTGACCGCGCCATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGTTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCATTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465