Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WAZ80_RS19900 Genome accession   NZ_CP147074
Coordinates   4137439..4138116 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain JSWP021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4132439..4143116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ80_RS19865 (WAZ80_19875) gspG 4132919..4133356 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  WAZ80_RS19870 (WAZ80_19880) gspH 4133364..4133873 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  WAZ80_RS19875 (WAZ80_19885) gspI 4133870..4134247 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  WAZ80_RS19880 (WAZ80_19890) gspJ 4134240..4134827 (+) 588 WP_001402582.1 type II secretion system minor pseudopilin GspJ -
  WAZ80_RS19885 (WAZ80_19895) gspK 4134820..4135803 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  WAZ80_RS19890 (WAZ80_19900) gspL 4135818..4136981 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  WAZ80_RS19895 (WAZ80_19905) gspM 4136978..4137439 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  WAZ80_RS19900 (WAZ80_19910) pilD 4137439..4138116 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  WAZ80_RS19905 (WAZ80_19915) bfr 4138145..4138621 (-) 477 WP_000675504.1 bacterioferritin -
  WAZ80_RS19910 (WAZ80_19920) bfd 4138693..4138887 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WAZ80_RS19915 (WAZ80_19925) chiA 4139056..4141749 (-) 2694 WP_001402584.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=858599 WAZ80_RS19900 WP_000178161.1 4137439..4138116(+) (pilD) [Escherichia coli strain JSWP021]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=858599 WAZ80_RS19900 WP_000178161.1 4137439..4138116(+) (pilD) [Escherichia coli strain JSWP021]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAACTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378