Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WAZ80_RS19265 Genome accession   NZ_CP147074
Coordinates   4023186..4024619 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain JSWP021     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4018186..4029619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ80_RS19235 (WAZ80_19245) yrbG 4018744..4019721 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  WAZ80_RS19240 (WAZ80_19250) kdsD 4019735..4020721 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  WAZ80_RS19245 (WAZ80_19255) kdsC 4020742..4021308 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WAZ80_RS19250 (WAZ80_19260) lptC 4021305..4021880 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  WAZ80_RS19255 (WAZ80_19265) lptA 4021849..4022406 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  WAZ80_RS19260 (WAZ80_19270) lptB 4022413..4023138 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  WAZ80_RS19265 (WAZ80_19275) rpoN/rpoN1 4023186..4024619 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  WAZ80_RS19270 (WAZ80_19280) hpf 4024642..4024929 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  WAZ80_RS19275 (WAZ80_19285) ptsN 4025047..4025538 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WAZ80_RS19280 (WAZ80_19290) rapZ 4025584..4026438 (+) 855 WP_000243741.1 RNase adapter RapZ -
  WAZ80_RS19285 (WAZ80_19295) npr 4026435..4026707 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  WAZ80_RS19290 (WAZ80_19300) yrbL 4026921..4027553 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  WAZ80_RS19295 (WAZ80_19305) mtgA 4027550..4028278 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  WAZ80_RS19300 (WAZ80_19310) elbB 4028275..4028928 (-) 654 WP_001307414.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=858596 WAZ80_RS19265 WP_000809051.1 4023186..4024619(+) (rpoN/rpoN1) [Escherichia coli strain JSWP021]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=858596 WAZ80_RS19265 WP_000809051.1 4023186..4024619(+) (rpoN/rpoN1) [Escherichia coli strain JSWP021]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTACTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465