Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WAZ54_RS08395 Genome accession   NZ_CP147056
Coordinates   1594081..1594758 (+) Length   225 a.a.
NCBI ID   WP_000178168.1    Uniprot ID   -
Organism   Escherichia coli strain 1002W03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1589081..1599758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ54_RS08360 (WAZ54_08365) gspG 1589561..1589998 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  WAZ54_RS08365 (WAZ54_08370) gspH 1590006..1590515 (+) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  WAZ54_RS08370 (WAZ54_08375) gspI 1590512..1590889 (+) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  WAZ54_RS08375 (WAZ54_08380) gspJ 1590882..1591469 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  WAZ54_RS08380 (WAZ54_08385) gspK 1591462..1592445 (+) 984 WP_001058515.1 type II secretion system minor pseudopilin GspK -
  WAZ54_RS08385 (WAZ54_08390) gspL 1592460..1593623 (+) 1164 WP_001296464.1 type II secretion system protein GspL -
  WAZ54_RS08390 (WAZ54_08395) gspM 1593620..1594081 (+) 462 WP_001385521.1 type II secretion system protein GspM -
  WAZ54_RS08395 (WAZ54_08400) pilD 1594081..1594758 (+) 678 WP_000178168.1 prepilin peptidase GspO Machinery gene
  WAZ54_RS08400 (WAZ54_08405) bfr 1594787..1595263 (-) 477 WP_000675503.1 bacterioferritin -
  WAZ54_RS08405 (WAZ54_08410) bfd 1595335..1595529 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WAZ54_RS08410 (WAZ54_08415) chiA 1595698..1598400 (-) 2703 WP_000773178.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24856.72 Da        Isoelectric Point: 7.6364

>NTDB_id=858236 WAZ54_RS08395 WP_000178168.1 1594081..1594758(+) (pilD) [Escherichia coli strain 1002W03]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLIHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=858236 WAZ54_RS08395 WP_000178168.1 1594081..1594758(+) (pilD) [Escherichia coli strain 1002W03]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGATTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTCATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387