Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I0P84_RS12830 Genome accession   NZ_AP024524
Coordinates   2675162..2675962 (-) Length   266 a.a.
NCBI ID   WP_005232134.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain RYU24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2670162..2680962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I0P84_RS12810 (RYU24_25420) - 2671213..2671911 (-) 699 WP_005232125.1 YfgM family protein -
  I0P84_RS12815 (RYU24_25430) hisS 2671942..2673237 (-) 1296 WP_104426613.1 histidine--tRNA ligase -
  I0P84_RS12820 (RYU24_25440) ispG 2673241..2674362 (-) 1122 WP_005232130.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I0P84_RS12825 (RYU24_25450) - 2674386..2675171 (-) 786 WP_214001583.1 helix-turn-helix domain-containing protein -
  I0P84_RS12830 (RYU24_25460) pilF 2675162..2675962 (-) 801 WP_005232134.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I0P84_RS12835 (RYU24_25470) rlmN 2675977..2677212 (-) 1236 WP_005232136.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I0P84_RS12840 (RYU24_25480) ndk 2677334..2677765 (-) 432 WP_004782097.1 nucleoside-diphosphate kinase -
  I0P84_RS12845 (RYU24_25490) iscX 2677875..2678075 (-) 201 WP_005232138.1 Fe-S cluster assembly protein IscX -
  I0P84_RS12850 (RYU24_25500) - 2678164..2678715 (-) 552 WP_004782094.1 phosphatase PAP2 family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29990.11 Da        Isoelectric Point: 9.0592

>NTDB_id=85816 I0P84_RS12830 WP_005232134.1 2675162..2675962(-) (pilF) [Acinetobacter variabilis strain RYU24]
MSNLTSKLALVSVVCGSLVLTACQTPDLGQKDPEKATKVRTQLAVEYLKNGDLDSAKRALDQALETNPRDSQANMMMGVL
LQQEGSKPNLEKAEGYFKRAISADPKNAQARNNYGTYLYQLERYKDAIEQFEIAGATLGYDQRFRALENMGRIYLKQGDL
VNAEKSFKQALQANRASYISMLELAEIFYLNQQFPAATQMYEQFVRGVGQKNQSARALWIGIRTARASGDQLGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=85816 I0P84_RS12830 WP_005232134.1 2675162..2675962(-) (pilF) [Acinetobacter variabilis strain RYU24]
TTGAGTAATCTGACATCTAAACTTGCTTTGGTTTCAGTAGTATGTGGGTCTTTGGTTTTAACTGCGTGTCAAACGCCAGA
TCTGGGTCAGAAGGACCCGGAAAAAGCCACCAAGGTTCGTACTCAGTTGGCGGTTGAATATCTTAAAAACGGTGATCTGG
ATTCAGCTAAACGTGCGCTGGATCAGGCACTGGAAACTAATCCGCGTGATTCGCAAGCCAATATGATGATGGGCGTGCTG
CTTCAGCAGGAAGGCAGCAAACCTAATCTTGAAAAGGCCGAAGGTTATTTCAAGCGTGCAATTTCGGCAGATCCCAAAAA
TGCCCAGGCACGAAATAACTACGGCACCTATTTATATCAGCTTGAGCGCTATAAAGATGCGATCGAGCAGTTCGAAATTG
CCGGCGCAACTTTAGGTTATGACCAGCGATTTCGTGCCCTTGAAAATATGGGACGGATTTACCTGAAACAGGGTGATTTG
GTGAATGCTGAAAAATCATTCAAACAGGCACTACAGGCTAATCGCGCTTCGTATATATCAATGTTAGAGTTGGCAGAAAT
TTTTTATCTGAACCAGCAGTTCCCGGCCGCGACTCAAATGTATGAACAGTTTGTACGTGGCGTGGGGCAGAAAAACCAGA
GTGCCCGTGCGCTCTGGATTGGCATTCGCACTGCGCGTGCCAGTGGCGATCAGTTAGGCATGCAGGTATTGGTCAATCAA
TTACGTGCATTGTTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(145-174)

Tetratricopeptide repeat (TPR_14)

(40-79)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.692

100

0.767
  pilF Acinetobacter baylyi ADP1

69.663

100

0.699

Multiple sequence alignment