Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QWJ31_RS23845 Genome accession   NZ_CP130340
Coordinates   3204664..3206142 (+) Length   492 a.a.
NCBI ID   WP_053821037.1    Uniprot ID   A0A849BDH7
Organism   Cupriavidus gilardii strain M5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3199664..3211142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QWJ31_RS23825 (QWJ31_23825) - 3201237..3202220 (+) 984 WP_053822970.1 SIS domain-containing protein -
  QWJ31_RS23830 (QWJ31_23830) lptC 3202229..3202855 (+) 627 WP_053821038.1 LPS export ABC transporter periplasmic protein LptC -
  QWJ31_RS23835 (QWJ31_23835) lptA 3202936..3203565 (+) 630 WP_053822969.1 lipopolysaccharide transport periplasmic protein LptA -
  QWJ31_RS23840 (QWJ31_23840) lptB 3203648..3204472 (+) 825 WP_006579161.1 LPS export ABC transporter ATP-binding protein -
  QWJ31_RS23845 (QWJ31_23845) rpoN/rpoN1 3204664..3206142 (+) 1479 WP_053821037.1 RNA polymerase factor sigma-54 Machinery gene
  QWJ31_RS23850 (QWJ31_23850) hpf 3206192..3206545 (+) 354 WP_053821036.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  QWJ31_RS23855 (QWJ31_23855) ptsN 3206925..3207380 (+) 456 WP_053821035.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QWJ31_RS23860 (QWJ31_23860) hprK 3207453..3208424 (+) 972 WP_006576781.1 HPr(Ser) kinase/phosphatase -
  QWJ31_RS23865 (QWJ31_23865) - 3208601..3208891 (+) 291 WP_053821034.1 PsiF family protein -
  QWJ31_RS23870 (QWJ31_23870) rapZ 3209128..3210009 (+) 882 WP_053821033.1 RNase adapter RapZ -
  QWJ31_RS23875 (QWJ31_23875) - 3210094..3210747 (+) 654 WP_053821032.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 54891.27 Da        Isoelectric Point: 5.0336

>NTDB_id=858038 QWJ31_RS23845 WP_053821037.1 3204664..3206142(+) (rpoN/rpoN1) [Cupriavidus gilardii strain M5]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNVQTATTTSPAEPA
APADSHADGTAAADEGYADGAGDDYGGDWSLDDFARRPQSDEDEKTPLQLRDAEPTLREYLMEQLTPLKISTRDKGLAIF
LIESLDDDGYLSASLEEILSELPEELEFELDEVHAMLTLLQSFDPPGVGARNAAECLSLQLRRIEHPQRELAMTIVRHHL
ELLAARDYTRLKKALQVDDMQLKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGHNWIAQLNPDVMPRLRINDMYA
QILRGAKGDTGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRV
TTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYREA
LKIPAVNLRKSL

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=858038 QWJ31_RS23845 WP_053821037.1 3204664..3206142(+) (rpoN/rpoN1) [Cupriavidus gilardii strain M5]
ATGAAACCGTCGCTACAGCTACGCCTCTCCCAGCATCTGGCCCTGACGCCGCAGCTGCAGCAGTCCATCCGGCTGTTGCA
GTTGTCCACGCTGGAACTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAATCCGCTGCTCGAACGCGAAAACGACTGGA
TCGAAAGCCCGCTGCGCGTTGCCGCCGACGGGTCGGTCAATGTGCAGACCGCCACCACGACCTCGCCCGCCGAACCGGCG
GCGCCCGCGGACTCGCACGCGGACGGCACCGCCGCAGCCGACGAAGGCTATGCCGACGGCGCGGGCGACGATTATGGCGG
CGACTGGAGCCTGGACGACTTCGCCCGCCGTCCGCAAAGCGATGAGGACGAGAAAACACCGCTGCAATTGCGCGATGCCG
AGCCCACGCTGCGCGAATATCTGATGGAGCAATTGACGCCGCTGAAAATCTCGACGCGCGACAAGGGCCTGGCGATTTTC
CTGATCGAATCGCTCGACGACGACGGTTATCTGAGCGCGTCGCTCGAGGAAATCCTGTCCGAATTGCCGGAAGAGCTCGA
ATTCGAACTCGACGAAGTGCATGCGATGCTGACGCTGCTGCAAAGCTTCGATCCGCCCGGGGTCGGCGCACGCAACGCAG
CAGAGTGCCTGTCGCTGCAACTGCGCCGCATCGAACATCCGCAGCGCGAGCTGGCGATGACCATCGTCCGCCATCACCTC
GAACTGCTGGCCGCGCGCGACTACACACGGCTGAAGAAGGCGCTGCAGGTCGACGACATGCAGCTGAAGGCCGCGCACGA
GTTGATCCGCTCGCTCGCGCCGTATCCCGGCCACGCCTACAGCCGGCCGGAAGCGGACTTCGTGGTGCCCGATGTATTCG
TGCGCAAGAGCGGCCACAACTGGATCGCCCAGCTCAATCCCGATGTGATGCCGCGGCTACGCATCAACGACATGTATGCG
CAGATCTTGCGCGGTGCGAAAGGCGATACCGGCGCCGCGGGATTGCAGCAGAAACTGCAGGAGGCGCGCTGGCTCATCAA
AAATATCCAGCAGCGTTTCGACACCATTTTGCGCGTCGCACAAGCCATCGTGGAGCGGCAAAAGAATTTCTTTACGCACG
GCGAAATTGCGATGCGCCCCTTGGTTTTGAGGGAGATAGCCGATACACTGGGTCTACACGAGTCGACTATTTCTCGTGTG
ACGACCAACAAGTACATGGCCACCCCGATGGGGACATTCGAGCTGAAATATTTTTTCGGCAGCCATGTATCGACCGAGAC
CGGCGGTGCCGCCTCTTCCACCGCAATCCGTGCGCTGATCAAGCAGCTGATAGGAGCCGAAGACCCCAGAAACCCGTTGT
CCGACAGCCGGATTGCCGAACTGCTGGGGGAACAAGGCTTCGTCGTGGCGCGACGTACCGTCGCCAAATATCGTGAAGCC
TTGAAGATCCCGGCCGTGAACCTTCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-319)

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-490)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A849BDH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

78.958

100

0.801