Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   Q2U85_RS27100 Genome accession   NZ_CP130335
Coordinates   5227727..5229076 (-) Length   449 a.a.
NCBI ID   WP_153588739.1    Uniprot ID   -
Organism   Bacillus cereus strain DW444     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5222727..5234076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q2U85_RS27065 (Q2U85_27065) - 5222773..5223468 (-) 696 WP_000466510.1 hypothetical protein -
  Q2U85_RS27070 (Q2U85_27070) - 5223455..5224243 (-) 789 WP_000120044.1 hypothetical protein -
  Q2U85_RS27075 (Q2U85_27075) - 5224271..5224840 (-) 570 WP_153588737.1 hypothetical protein -
  Q2U85_RS27080 (Q2U85_27080) - 5224958..5225431 (-) 474 WP_258112912.1 hypothetical protein -
  Q2U85_RS27085 (Q2U85_27085) raiA 5225835..5226377 (-) 543 WP_000671186.1 ribosome-associated translation inhibitor RaiA -
  Q2U85_RS27090 (Q2U85_27090) cspC 5226699..5226896 (-) 198 WP_001990088.1 cold shock protein CspC -
  Q2U85_RS27095 (Q2U85_27095) - 5227023..5227727 (-) 705 WP_153588738.1 ComF family protein -
  Q2U85_RS27100 (Q2U85_27100) comFA 5227727..5229076 (-) 1350 WP_153588739.1 ATP-dependent helicase ComFA Machinery gene
  Q2U85_RS27105 (Q2U85_27105) - 5229204..5230631 (-) 1428 WP_153588740.1 NlpC/P60 family protein -
  Q2U85_RS27110 (Q2U85_27110) - 5230780..5231094 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  Q2U85_RS27115 (Q2U85_27115) - 5231267..5232109 (-) 843 WP_000684725.1 DegV family protein -
  Q2U85_RS27120 (Q2U85_27120) - 5232346..5232981 (+) 636 WP_258112911.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51050.06 Da        Isoelectric Point: 10.1031

>NTDB_id=858018 Q2U85_RS27100 WP_153588739.1 5227727..5229076(-) (comFA) [Bacillus cereus strain DW444]
MLAGKQLLLEELSSNLQRKLNDLKKKGEIVCVQGVKKKASKYMCQRCGNVDRRLFASFLCKRCSKVCAYCRKCITMGRVS
ECTVLVRGIAERKREKNLNLLQWNGTLSTGQNLAAQGVIEAIRQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=858018 Q2U85_RS27100 WP_153588739.1 5227727..5229076(-) (comFA) [Bacillus cereus strain DW444]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAAATTTACAGAGGAAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGGCTTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCGCATATTGCCGGAAGTGTATAACGATGGGGAGAGTAAGT
GAATGTACTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTTAAACTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAGGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTTGTTGCGACTACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCGTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCATCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCTATTTTTTTA
TTCGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCAGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-408)

DEAD/DEAH box helicase (DEAD)

(117-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.914

93.096

0.483
  comFA Latilactobacillus sakei subsp. sakei 23K

41.439

89.755

0.372