Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   Q2U85_RS20955 Genome accession   NZ_CP130335
Coordinates   4101265..4101699 (-) Length   144 a.a.
NCBI ID   WP_016111238.1    Uniprot ID   A0A9W5PPJ2
Organism   Bacillus cereus strain DW444     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4096265..4106699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q2U85_RS20940 (Q2U85_20940) - 4097443..4098000 (+) 558 WP_000062063.1 PadR family transcriptional regulator -
  Q2U85_RS20945 (Q2U85_20945) metE 4098512..4100800 (+) 2289 WP_302720384.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  Q2U85_RS20950 (Q2U85_20950) comJ 4100839..4101228 (-) 390 WP_016111237.1 competence protein ComJ -
  Q2U85_RS20955 (Q2U85_20955) nucA/comI 4101265..4101699 (-) 435 WP_016111238.1 DNA-entry nuclease Machinery gene
  Q2U85_RS20960 (Q2U85_20960) - 4101926..4102675 (+) 750 WP_153587766.1 DUF3967 domain-containing protein -
  Q2U85_RS20965 (Q2U85_20965) kinB 4102714..4103988 (-) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  Q2U85_RS20970 (Q2U85_20970) - 4104299..4104499 (+) 201 WP_000929277.1 hypothetical protein -
  Q2U85_RS20975 (Q2U85_20975) - 4104622..4105785 (+) 1164 WP_000434578.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16053.45 Da        Isoelectric Point: 8.9015

>NTDB_id=858004 Q2U85_RS20955 WP_016111238.1 4101265..4101699(-) (nucA/comI) [Bacillus cereus strain DW444]
MKQLKGIIISIIAILSILVAVYEVLVPQETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=858004 Q2U85_RS20955 WP_016111238.1 4101265..4101699(-) (nucA/comI) [Bacillus cereus strain DW444]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTGGCAGTTTATGAAGTACTTGTGCC
ACAGGAAACAAGTGTAAAGAAAACAAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACCGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.085

81.25

0.569