Detailed information NTG0857983

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: Q2U85_RS06285
• Genome accession: NZ_CP130335
• Coordinates: 1192460..1196185 (+)
• Length: 1241 a.a.
• NCBI ID: WP_153587954.1
• Uniprot ID: -
• Organism: Bacillus cereus strain DW444
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1187460..1201185

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q2U85_RS06270 (Q2U85_06270)	-	1187618..1188211 (+)	594	WP_000347518.1	TVP38/TMEM64 family protein	-
Q2U85_RS06275 (Q2U85_06275)	lepB	1188268..1188831 (+)	564	WP_000751910.1	signal peptidase I	-
Q2U85_RS06280 (Q2U85_06280)	addB	1188948..1192463 (+)	3516	WP_153587955.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
Q2U85_RS06285 (Q2U85_06285)	addA	1192460..1196185 (+)	3726	WP_153587954.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
Q2U85_RS06290 (Q2U85_06290)	-	1196198..1196485 (+)	288	WP_000255727.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
Q2U85_RS06295 (Q2U85_06295)	gerPF	1196623..1196838 (-)	216	WP_001141566.1	spore germination protein GerPF	-
Q2U85_RS06300 (Q2U85_06300)	-	1196881..1197267 (-)	387	WP_000902319.1	spore germination protein GerPE	-
Q2U85_RS06305 (Q2U85_06305)	gerPD	1197283..1197477 (-)	195	WP_001052807.1	spore germination protein GerPD	-
Q2U85_RS06310 (Q2U85_06310)	gerPC	1197484..1198098 (-)	615	WP_001070760.1	spore germination protein GerPC	-
Q2U85_RS06315 (Q2U85_06315)	gerPB	1198166..1198372 (-)	207	WP_001012508.1	spore germination protein GerPB	-
Q2U85_RS06320 (Q2U85_06320)	gerPA	1198387..1198608 (-)	222	WP_001111187.1	spore germination protein GerPA	-
Q2U85_RS06325 (Q2U85_06325)	-	1198705..1198884 (-)	180	WP_000462845.1	aspartyl-phosphate phosphatase Spo0E family protein	-
Q2U85_RS06330 (Q2U85_06330)	-	1199131..1200030 (+)	900	WP_001213086.1	fumarylacetoacetate hydrolase family protein	-
Q2U85_RS06335 (Q2U85_06335)	-	1200066..1200350 (-)	285	WP_000926864.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142614.62 Da
• Isoelectric Point: 4.7561

>NTDB_id=857983 Q2U85_RS06285 WP_153587954.1 1192460..1196185(+) (addA) [Bacillus cereus strain DW444]
MIENWPEKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMIPDGPAPRIETLQADLALLGTLSSAAHESWTSLYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKNDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLSDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKDEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEDGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGNSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=857983 Q2U85_RS06285 WP_153587954.1 1192460..1196185(+) (addA) [Bacillus cereus strain DW444]
ATGATAGAAAATTGGCCTGAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAATGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGGTCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACCGAATC
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAACACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGATTCCTGACGGTCCAGCGCCTCGCATTGAAACCCTGCAAGCAGATCTAGCTTTACT
TGGAACGCTATCATCAGCTGCTCATGAGTCGTGGACAAGTTTGTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGAAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTTTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTTGTACAGGAATCTATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCCG
AACCAGGACTATTCCTAGGAAAATATAAACGCTTCACACAAGAAGGATTGGGCGGTGGAATGAAGATCGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
CGACTACGATGCAGACGCTGAATTAAAGTTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCAGAACTACTGTGCA
TTCAGCAAACGGAAGAAGAAGTAATAGACGGAGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGACCGTAAAAATGATAGTATGCGCCCAGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCCCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCAG
TGTACGCTGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCTCCAATCGTTGGATTGAGCGATGAAGAACTTGCAACGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCACTTGAAGAGGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGCGCAAGACAATACGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGACATGGGTACGGCGAGGGCCCTCGGTGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCGGTTGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTATTGCATAAAGATTTCGGTTTCGGTTCGCAATTTATTGATCCGCGTAAACGAATTAAATATACGACATTAT
CACAGCTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCTTTAACGCGT
GCAAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTCGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGGGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGATGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTGCCGTTAGAAAGTGAACGAAAAGATGAAGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCACCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCAGAAGATGGTAGTGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTTCCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAATAGCGGGGAATCGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTTCCAGGCGGATTCGAACAAGCGAAACCAATTTTA
GAAGATCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTGGAGAAAAGCTTACAACATCCTGTAAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.488	100	0.537