Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WAZ59_RS18215 Genome accession   NZ_CP147032
Coordinates   3654160..3654837 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 22F46     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3649160..3659837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAZ59_RS18200 (WAZ59_18200) chiA 3650527..3653220 (+) 2694 WP_020239449.1 bifunctional chitinase/lysozyme -
  WAZ59_RS18205 (WAZ59_18205) bfd 3653389..3653583 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WAZ59_RS18210 (WAZ59_18210) bfr 3653655..3654131 (+) 477 WP_000675504.1 bacterioferritin -
  WAZ59_RS18215 (WAZ59_18215) pilD 3654160..3654837 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  WAZ59_RS18220 (WAZ59_18220) gspM 3654837..3655298 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  WAZ59_RS18225 (WAZ59_18225) gspL 3655295..3656458 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  WAZ59_RS18230 (WAZ59_18230) gspK 3656473..3657456 (-) 984 WP_021570243.1 type II secretion system minor pseudopilin GspK -
  WAZ59_RS18235 (WAZ59_18235) gspJ 3657449..3658036 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  WAZ59_RS18240 (WAZ59_18240) gspI 3658029..3658406 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  WAZ59_RS18245 (WAZ59_18245) gspH 3658403..3658912 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  WAZ59_RS18250 (WAZ59_18250) gspG 3658920..3659357 (-) 438 WP_025269878.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=857692 WAZ59_RS18215 WP_000178161.1 3654160..3654837(-) (pilD) [Escherichia coli strain 22F46]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=857692 WAZ59_RS18215 WP_000178161.1 3654160..3654837(-) (pilD) [Escherichia coli strain 22F46]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378