Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   V9L10_RS20590 Genome accession   NZ_CP147025
Coordinates   4251264..4251941 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain KCTC 2571     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4246264..4256941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9L10_RS20555 (V9L10_20555) gspG 4246744..4247181 (+) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -
  V9L10_RS20560 (V9L10_20560) gspH 4247189..4247698 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  V9L10_RS20565 (V9L10_20565) gspI 4247695..4248072 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  V9L10_RS20570 (V9L10_20570) gspJ 4248065..4248652 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  V9L10_RS20575 (V9L10_20575) gspK 4248645..4249628 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  V9L10_RS20580 (V9L10_20580) gspL 4249643..4250806 (+) 1164 WP_001115235.1 type II secretion system protein GspL -
  V9L10_RS20585 (V9L10_20585) gspM 4250803..4251264 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  V9L10_RS20590 (V9L10_20590) pilD 4251264..4251941 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  V9L10_RS20595 (V9L10_20595) bfr 4251970..4252446 (-) 477 WP_000675504.1 bacterioferritin -
  V9L10_RS20600 (V9L10_20600) bfd 4252518..4252712 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  V9L10_RS20605 (V9L10_20605) chiA 4252881..4255574 (-) 2694 WP_000773142.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=857622 V9L10_RS20590 WP_000178161.1 4251264..4251941(+) (pilD) [Escherichia coli strain KCTC 2571]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=857622 V9L10_RS20590 WP_000178161.1 4251264..4251941(+) (pilD) [Escherichia coli strain KCTC 2571]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378