Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WA132_RS12370 Genome accession   NZ_CP146939
Coordinates   2598618..2600051 (-) Length   477 a.a.
NCBI ID   WP_001764900.1    Uniprot ID   -
Organism   Escherichia coli strain 2A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2593618..2605051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WA132_RS12335 (WA132_12335) elbB 2594310..2594963 (+) 654 WP_148983561.1 isoprenoid biosynthesis glyoxalase ElbB -
  WA132_RS12340 (WA132_12340) mtgA 2594960..2595688 (+) 729 WP_001764899.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  WA132_RS12345 (WA132_12345) yrbL 2595685..2596317 (-) 633 WP_000620407.1 PhoP regulatory network protein YrbL -
  WA132_RS12350 (WA132_12350) npr 2596530..2596802 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  WA132_RS12355 (WA132_12355) rapZ 2596799..2597653 (-) 855 WP_000243741.1 RNase adapter RapZ -
  WA132_RS12360 (WA132_12360) ptsN 2597699..2598190 (-) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WA132_RS12365 (WA132_12365) hpf 2598308..2598595 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  WA132_RS12370 (WA132_12370) rpoN/rpoN1 2598618..2600051 (-) 1434 WP_001764900.1 RNA polymerase factor sigma-54 Machinery gene
  WA132_RS12375 (WA132_12375) lptB 2600099..2600824 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  WA132_RS12380 (WA132_12380) lptA 2600831..2601388 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  WA132_RS12385 (WA132_12385) lptC 2601357..2601932 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  WA132_RS12390 (WA132_12390) kdsC 2601929..2602495 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WA132_RS12395 (WA132_12395) kdsD 2602516..2603502 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  WA132_RS12400 (WA132_12400) yrbG 2603516..2604493 (-) 978 WP_000922880.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53992.81 Da        Isoelectric Point: 4.3837

>NTDB_id=857374 WA132_RS12370 WP_001764900.1 2598618..2600051(-) (rpoN/rpoN1) [Escherichia coli strain 2A]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGNEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=857374 WA132_RS12370 WP_001764900.1 2598618..2600051(-) (rpoN/rpoN1) [Escherichia coli strain 2A]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCAATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCTGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGCGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465